CAJ | 학술논문

目的：建立昆仑雪菊中木犀草素的含量测定方法。方法采用反相高效液相色谱法测定，色谱柱：Waters XBridge C18（4．6 mm ×250 mm，5μm），流动相 A：乙腈；B：2％冰醋酸；C：水；梯度洗脱程序：0 min，A∶B （15∶85）；10 min，A∶B （20∶80）；15 min，A∶B（35∶65）；30 min，A∶B（15∶85），柱温35℃，检测波长350 nm，流速1．0 mL/min。结果木犀草素在0．1～1．0μg/mL浓度范围内线性关系良好（r ＝0．9999），平均回收率为94．90％，RSD为0．69％。结论该方法简便，结果准确，可以用于昆仑雪菊中木犀草素的含量测定。
목적：건립곤륜설국중목서초소적함량측정방법。방법채용반상고효액상색보법측정，색보주：Waters XBridge C18（4．6 mm ×250 mm，5μm），류동상 A：을정；B：2％빙작산；C：수；제도세탈정서：0 min，A∶B （15∶85）；10 min，A∶B （20∶80）；15 min，A∶B（35∶65）；30 min，A∶B（15∶85），주온35℃，검측파장350 nm，류속1．0 mL/min。결과목서초소재0．1～1．0μg/mL농도범위내선성관계량호（r ＝0．9999），평균회수솔위94．90％，RSD위0．69％。결론해방법간편，결과준학，가이용우곤륜설국중목서초소적함량측정。
Objective To develop an HPLC method for the determination of the content of luteolin in Kun-lun chrysanthemum.Methods The content of luteolin in Kunlun chrysanthemum was determined by HPLC.The exact process was:column:Waters XBridge C18(250 mm×4.6 mm,5μm);mobile phase A:cetonitrile;B:2%glacial acetic acid(V/V),gradient elution:0 min,A∶B(15∶85);10 min,A∶B(20∶80);15 min,A∶B(35∶65);30 min,A∶B(15∶85);the flow rate was 1.0 mL/min,the detection wave-length was at 350 nm,and the column temperature was 35℃.Results Linearity of luteolin was good in the range of 0.1~1μg/mL (r=0.999 9).The average recovery of luteolin was 94.90% with RSD 0.69%. Conclusion This method is convenient,accurate,and can be used to determine the content of luteolin in Kunlun chrysanthemum.