CAJ | 학술논문

目的：观察茯苓多糖体外对大鼠精子膜功能、精子 DNA 和存活力的保护作用。方法取大鼠精子制备精子悬液，并分为正常组（PBS），生理盐水组，阳性药对照组（维生素 C 组）和茯苓多糖低、中、高剂量组（0.5mg/ml，1.0mg/ml 和5.0mg/ml）。各对照物及茯苓多糖分别与精子悬液共同孵育后，检测精子膜完整性、DNA 损伤程度及存活力。结果低、中、高浓度的茯苓多糖在相同的条件下均可增加精子存活时间，提高精子存活率和活力，降低精子 DNA 损伤比率，对精子膜功能和 DNA 均具有一定的保护作用。其中1.0mg/ml 和5.0mg/ml（中、高）的茯苓多糖组作用明显优于维生素 C 组( P <0.05或 P <0.01)。结论茯苓多糖对大鼠精子膜和 DNA 有明显的保护作用，并可延长精子的体外存活时间。
목적：관찰복령다당체외대대서정자막공능、정자 DNA 화존활력적보호작용。방법취대서정자제비정자현액，병분위정상조（PBS），생리염수조，양성약대조조（유생소 C 조）화복령다당저、중、고제량조（0.5mg/ml，1.0mg/ml 화5.0mg/ml）。각대조물급복령다당분별여정자현액공동부육후，검측정자막완정성、DNA 손상정도급존활력。결과저、중、고농도적복령다당재상동적조건하균가증가정자존활시간，제고정자존활솔화활력，강저정자 DNA 손상비솔，대정자막공능화 DNA 균구유일정적보호작용。기중1.0mg/ml 화5.0mg/ml（중、고）적복령다당조작용명현우우유생소 C 조( P <0.05혹 P <0.01)。결론복령다당대대서정자막화 DNA 유명현적보호작용，병가연장정자적체외존활시간。
Objective To explore the protective effect of pachyman from Poria cocos on rat sperm membrane, sperm DNA and viability in vitro. Methods Rat sperm suspension was prepared in PBS, and divided into normal group (PBS), and normal saline group, positive control group (vitamin C) and crude extractings from poria cocos of the low, medium and high dose group (0.5mg/ml,1.0mg/ml and 5.0 mg/ml). After the pachyman was incubated with the sperm suspension, DNA damaged sperm and sperm membrane integrity were detected by the improved Eosin-Y staining, hypo-osmotic swelling test and the sperm chromatin dispersion test. Results The low, medium and high dosage of pachyman under the same incubated conditions could prolong sperm survival time and improve the sperm vitality, reduce the percentage of sperm DNA damage in vitro. Of which, 1.0 mg/ml and 5.0 mg/ml (middle, high) of pachyman group were significantly better than vitamin C group (P < 0.05 or P < 0.01). Conclusions The pachyman had significant protective effect on rate sperm membrane and DNA.