CAJ | 학술논문

目的：探讨高效液相色谱串联质谱（HPLC-MS/MS）法测定人血浆中瑞巴派特浓度的不确定度评定方法。方法用HPLC-MS/MS法测定人血浆中瑞巴派特浓度，通过对测试方法过程进行分析，确定不确定度来源，计算合成不确定度和扩展不确定度。结果血浆中瑞巴派特浓度测定的合成不确定度为4.83%，扩展不确定度为9.66％。结论本评定方法适用于HPLC-MS/MS法测定人血浆中瑞巴派特浓度的不确定度评定，确立不确定度的评定方法对于血药浓度的测定具有重要意义。
목적：탐토고효액상색보천련질보（HPLC-MS/MS）법측정인혈장중서파파특농도적불학정도평정방법。방법용HPLC-MS/MS법측정인혈장중서파파특농도，통과대측시방법과정진행분석，학정불학정도래원，계산합성불학정도화확전불학정도。결과혈장중서파파특농도측정적합성불학정도위4.83%，확전불학정도위9.66％。결론본평정방법괄용우HPLC-MS/MS법측정인혈장중서파파특농도적불학정도평정，학립불학정도적평정방법대우혈약농도적측정구유중요의의。
Objective To evaluate the uncertainty of the determination of rebamipide in human plasma by HPLC-MS/MS. Methods The content of rebamipide in human plasma was determined by HPLC-MS/MS. Uncertainty sources were obtained from analysis of the whole procedure of HPLC-MS/MS method. The combined standard uncertainty and expanded standard uncertainty were calculated. Results The combined standard uncertainty and expanded standard uncertainty of the determination of rebamipide concentration in human plasma was 4.83% and 9.66%, respectively. Conclusion The method is suitable for uncertainty evaluation of the determination of rebamipide in human plasma by HPLC-MS/MS method. The evaluation of measurement uncertainty is important to the determination of drug concentration in plasma.