CAJ | 학술논문

目的：探讨乳腺癌细胞化疗继发耐药后细胞迁移能力的变化，并筛选出相关基因。方法以MCF-7细胞株为亲本细胞，采用阿霉素（ADM）低浓度持续加量诱导法建立对ADM耐药的人乳腺癌细胞株MCF-7/ADM。应用ATP- TCA药物敏感检测法和xCELLIigence/RT- CES实时细胞电子分析系统检测人乳腺癌细胞株MCF-7、MCF-7/ADM对多种化疗药物的耐药情况。应用Transwel 实验和xCELLIigence/RT- CIM实时细胞电子分析系统检测人乳腺癌细胞株MCF-7、MCF-7/ADM的细胞迁移能力。应用比较基因组芯片筛选出人乳腺癌细胞株 MCF-7、MCF-7/ADM DNA 拷贝数4倍差异的基因。结果撤药培养150d 后， MCF-7/ADM细胞较亲本细胞MCF-7的ADM耐药指数为72.9倍，对其他化疗药物无明显的交叉耐药性。MCF-7/ADM细胞迁移能力较MCF-7明显降低（P＜0.05）。MCF-7/ADM有12种基因DNA拷贝数是MCF-7的4倍，MCF-7有6种基因DNA拷贝数是MCF-7/ADM的4倍。结论乳腺癌细胞化疗继发耐药伴随细胞迁移能力明显降低，其相关基因的DNA拷贝数亦有明显差异。
목적：탐토유선암세포화료계발내약후세포천이능력적변화，병사선출상관기인。방법이MCF-7세포주위친본세포，채용아매소（ADM）저농도지속가량유도법건립대ADM내약적인유선암세포주MCF-7/ADM。응용ATP- TCA약물민감검측법화xCELLIigence/RT- CES실시세포전자분석계통검측인유선암세포주MCF-7、MCF-7/ADM대다충화료약물적내약정황。응용Transwel 실험화xCELLIigence/RT- CIM실시세포전자분석계통검측인유선암세포주MCF-7、MCF-7/ADM적세포천이능력。응용비교기인조심편사선출인유선암세포주 MCF-7、MCF-7/ADM DNA 고패수4배차이적기인。결과철약배양150d 후， MCF-7/ADM세포교친본세포MCF-7적ADM내약지수위72.9배，대기타화료약물무명현적교차내약성。MCF-7/ADM세포천이능력교MCF-7명현강저（P＜0.05）。MCF-7/ADM유12충기인DNA고패수시MCF-7적4배，MCF-7유6충기인DNA고패수시MCF-7/ADM적4배。결론유선암세포화료계발내약반수세포천이능력명현강저，기상관기인적DNA고패수역유명현차이。
Objective To investigate the change of the cellmigration ability of breast cancer cells with acquired resistance to chemotherapy, and screen the related genes. Methods MCF- 7 cells were used as the parental cells, and human breast can-cer cellline MCF- 7/ADM were established with continuous low concentration of ADM. The chemotherapy resistance of MCF- 7 and MCF- 7/ADM cellwas detected by ATP- TCA drug sensitive test and xCELLIigence/RT- CES real- time cellelectronic analysis system. The cellmigration of MCF- 7 and MCF- 7/ADM cellwas detected by Transwel test and xCELLIigence/RT- CIM real- time cellelectronic analysis system. 4 times the difference of DNA copies of Genes in MCF- 7 and MCF- 7/ADM cellwere screeninged by comparative genomic chip. Results The drug resistant index of MCF- 7/ADM cellkept on 72.9 times over MCF- 7 cellafter cultured in the medium (without ADM) for 150 days. MCF- 7/ADM had no obvious cross- resistance to other chemotherapy drugs. The cellmigration of MCF- 7/ADM reduced significantly than that of MCF- 7 (P<0.05). The DNA copies of 12 genes of MCF- 7/ADM cellare 4 times more than that of MCF- 7, while the DNA copies of 6 genes of MCF- 7 cell are 4 times more than that of MCF- 7/ADM. Conclusion The migration ability of breast cancer cells was significantly reduced with acquired chemotherapy resistance,and the DNA copies of some genes are also obvious different.