CAJ | 학술논문

目的：探讨胃癌组织中自噬相关蛋白Beclin1与微管相关蛋白轻链3（MAPLC3）的表达及其临床意义。方法采用免疫组化En- Vision法检测59例胃癌组织和相应的癌旁组织中Beclin1及MAPLC3的表达水平，分析其与多种胃癌临床病理参数之间的关系。结果在胃癌组织中Beclin1和MAPLC3的阳性表达率分别为40.7%和44.1%,癌旁组织的阳性表达率分别为100%、93.2%，癌组织明显低于癌旁组织（P＜0.05）；Beclin1和MAPLC3的表达水平与胃癌的浸润深度、淋巴结转移及TNM分期相关（P＜0.05）；胃癌组织中Beclin1和MAPLC3的表达水平呈正相关（r=0.425，P＜0.05）。结论胃癌组织中Beclin1和MAPLC3表达水平均明显低于癌旁组织，且两者之间呈正相关，两者的低表达可能共同促进胃癌的淋巴结转移。
목적：탐토위암조직중자서상관단백Beclin1여미관상관단백경련3（MAPLC3）적표체급기림상의의。방법채용면역조화En- Vision법검측59례위암조직화상응적암방조직중Beclin1급MAPLC3적표체수평，분석기여다충위암림상병리삼수지간적관계。결과재위암조직중Beclin1화MAPLC3적양성표체솔분별위40.7%화44.1%,암방조직적양성표체솔분별위100%、93.2%，암조직명현저우암방조직（P＜0.05）；Beclin1화MAPLC3적표체수평여위암적침윤심도、림파결전이급TNM분기상관（P＜0.05）；위암조직중Beclin1화MAPLC3적표체수평정정상관（r=0.425，P＜0.05）。결론위암조직중Beclin1화MAPLC3표체수평균명현저우암방조직，차량자지간정정상관，량자적저표체가능공동촉진위암적림파결전이。
Objective To investigate the expression of Beclin1 and MAPLC3 in gastric cancer and clinical significance. Methods Immunohistochemical assay was performed to detect the expression of Beclin1 and MAPLC3 in fifty- nine gastric carci-noma tissues and their adjacent noncancerous specimens. Results (1) The expression of Beclin1 and MAPLC3 was both de-creased in carcinoma tissues(24/59, 40.7%and 26/59, 44.1%), which were significantly lower than those in noncancerous tissues (P<0.05).(2)The expression of both Beclin1 and MAPLC3 was correlated with depth of invasion, lymph node metastasis and TNM classification (P<0.05).(3) There was positive correlation between the expression of Beclin1 and MAPLC3 (r=0.425, P<0.05). Conclusion The expression of Beclin1 and MAPLC3 both reduced in gastric carcinoma tissues, and the downregulation of Beclin1 is significantly associated with MAPLC3. The co- downregulation of Beclin1 and MAPLC3 may play an important role in lymphatic spread of gastric carcinoma.