CAJ | 학술논문

目的：探讨使用E H-2060B型全自动尿沉渣分析仪在临床尿分析检查中的应用价值，分析各种尿液分析方法所致结果差异的原因及解决方法。方法按中国临床实验室标准化委员会有关规定和《尿沉渣检查标准化的建议》要求随机收集1912例尿液标本，使用E H-2060B型尿沉渣分析仪和干化学法进行红细胞（RBC ）、白细胞（WBC ）等有形成分的分析。结果以尿沉渣镜检法为基础进行评价，干化学法检测 RBC、WBC 假阴性率为16．8％、22．8％，假阳性率为11．5％、5．7％，均高于 E H-2060B分析仪假阴性率15．1％、17．2％，假阳性率3．2％、2．1％，两种方法除了尿RBC的假阴性率差异无统计学意义（ P＞0．05），其余指标差异均有统计学意义（ P＜0．05）。以镜检法为参考方法，EH-2060B尿沉渣仪检测RBC、WBC总符合率及一致性检验Kappa值均高于干化学法。干化学法与尿沉渣仪联合检测，RBC假阴性率为2．0％，WBC假阴性率为3．9％。结论与干化学法比较，EH-2060B型尿沉渣分析仪更方便、快捷、结果可靠，但也存在一定局限性，不能完全替代人工镜检，只能作为筛选指标，两种方法应联合检测，互为补充，并以显微镜镜检法为金标准，提高尿液有形成分的检出率。
목적：탐토사용E H-2060B형전자동뇨침사분석의재림상뇨분석검사중적응용개치，분석각충뇨액분석방법소치결과차이적원인급해결방법。방법안중국림상실험실표준화위원회유관규정화《뇨침사검사표준화적건의》요구수궤수집1912례뇨액표본，사용E H-2060B형뇨침사분석의화간화학법진행홍세포（RBC ）、백세포（WBC ）등유형성분적분석。결과이뇨침사경검법위기출진행평개，간화학법검측 RBC、WBC 가음성솔위16．8％、22．8％，가양성솔위11．5％、5．7％，균고우 E H-2060B분석의가음성솔15．1％、17．2％，가양성솔3．2％、2．1％，량충방법제료뇨RBC적가음성솔차이무통계학의의（ P＞0．05），기여지표차이균유통계학의의（ P＜0．05）。이경검법위삼고방법，EH-2060B뇨침사의검측RBC、WBC총부합솔급일치성검험Kappa치균고우간화학법。간화학법여뇨침사의연합검측，RBC가음성솔위2．0％，WBC가음성솔위3．9％。결론여간화학법비교，EH-2060B형뇨침사분석의경방편、쾌첩、결과가고，단야존재일정국한성，불능완전체대인공경검，지능작위사선지표，량충방법응연합검측，호위보충，병이현미경경검법위금표준，제고뇨액유형성분적검출솔。
Objective To investigate the application value of EH-2060B automatic urinary sediment analyzer in clinic ,to find out the reason what induce results differing from other methods and the solution .Methods A total of 1 912 urine samples were collected randomly under the requirements of CCCLS (China Committee for Clinical Labora-tory Standards) and "Proposal of standardization for the Urine Sediment Exam",then analyzed the erythrocyte and leucocyte by urine dipsticks analyzer and EH-2060B automatic urinary sediment analyzer respectively .Results Refer-ring to the microscopic examination ,the false negative rate and the false positive rate of analyzing the erythrocyte and leucocyte by dry chemistry(false negative rate :16 .8% and 22 .8% ,the false positive rate :11 .5% and 5 .7% ) were both higher than those of EH-2060B (false negative rate :15 .1% and 17 .2% ,the false positive rate :3 .2% and 2 .1% ) ,Except the false negative rate of the analyzing the erythrocyte ,there were significant difference in the other index between the urine dipsticks analyzer and EH-2060B automatic urinary sediment analyzer (P<0 .05) .To com-pare the total coincidence and consistency with manual microscope ,the testing Kappa value of EH2060-B were higher than dry chemistry .Combined dry chemistry and sediment analyzer ,the false negative rate for RBC and WBC was im-proved to 2 .0% and 3 .9% .Conclusion Compared to the urine dipsticks analyzer ,EH-2060B analyzer is rapid ,simple and of high coincidence with manual microscopy ,however ,there are certain limitations ,it can′t replace the micro-scope examination at all ,and only as the preliminary screening .These two analysis methods could be complementary each other while taking manual microscopy as the golden criterion so as to gradually raise the positive coincidence of urinary sediment .