CAJ | 학술논문

目的：探讨乙肝转阴散的微生物限度分析和检查方法的有效性。方法选择5种菌为阳性对照，采用平皿计数法，采用回收率试验对细菌、丝状真菌及酵母菌计数方法的回收率进行验证；采用相同的实验条件，观察大肠埃希菌、大肠菌群、沙门菌在试验组、阳性对照组和阴性对照组的检出情况验证控制菌检查方法。结果经方法学验证试验得出，乙肝转阴散有抑菌作用，其微生物限度检查计数方法采用培养基稀释法，控制菌检查采用常规法。结论验证后的微生物限度检查方法简便可行、准确无误，适合于乙肝转阴散的微生物限度检查。
목적：탐토을간전음산적미생물한도분석화검사방법적유효성。방법선택5충균위양성대조，채용평명계수법，채용회수솔시험대세균、사상진균급효모균계수방법적회수솔진행험증；채용상동적실험조건，관찰대장애희균、대장균군、사문균재시험조、양성대조조화음성대조조적검출정황험증공제균검사방법。결과경방법학험증시험득출，을간전음산유억균작용，기미생물한도검사계수방법채용배양기희석법，공제균검사채용상규법。결론험증후적미생물한도검사방법간편가행、준학무오，괄합우을간전음산적미생물한도검사。
Objective To establish an examination method for microbial limit determination of Yiganzhuanyin powders .Methods Plate counting method was used .The method of counting bacteria and mould was validated by the recovery rates with 5 control trains .The method of checking control bacteria was validated by observing cultivation of Escherichia coli、coliform group and salmonella in the test group、positive control group and negative control group under the same environment .Results The results of validation test showed that Yiganzhuanyin powders had antibac-terial activity .The culture media dilution method was used as the counting methods for the bacteria detection can be carried out by the conwentional examination method .Conclusion The method was simple ,feasible ,reliable and could be used for the examination of microbacteria limit .