检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2013年
24期
3279-3280
,共2页
血清a-l-岩藻糖苷酶%高尔基体糖蛋白%甲胎蛋白%原发性肝癌%联合检测
血清a-l-巖藻糖苷酶%高爾基體糖蛋白%甲胎蛋白%原髮性肝癌%聯閤檢測
혈청a-l-암조당감매%고이기체당단백%갑태단백%원발성간암%연합검측
serum a-l-glycosidase%golgi glycoprotein%alpha-fetoprotein%primary hepatic carcinoma%combined detection
目的：探讨血清a-l-岩藻糖苷酶（AFU）、高尔基体糖蛋白73（GP73）和甲胎蛋白（AFP）在原发性肝癌（PHC）诊断中的临床意义。方法采用化学比色速率法、酶联免疫吸附试验和直接化学发光法测定32例PHC患者、42例肝良性疾病患者、50例健康对照者血清AFU、GP73和AFP水平，并进行比较。结果 PHC组患者血清AFU、GP73、AFP水平[（789．4±475．2）mol/L、（205．5±50．8）μg/L、（977．3±637．4）ng/mL]高于肝良性疾病组[（378．3±127．9）mol/L、（77．5±28．9）μg/L、（6．09±1．57）ng/mL]和健康对照组[（347．8±119．6）mol/L、（33．5±7．5）μg/L、（4．17±1．26）ng/mL]，差异有统计学意义（P＜0．01）。以血清AFU、GP73、AFP水平为指标，检测PHC患者的敏感性、特异性分别为75．1％、73．4％、62．2％；86．8％、67．5％、97．8％，3项联合检测的敏感性、特异性分别为93．4％、85．4％。结论3项指标联合检测，可显著增加阳性检出率，有助于PHC的诊断、病情变化判别及治疗效果观察。
目的：探討血清a-l-巖藻糖苷酶（AFU）、高爾基體糖蛋白73（GP73）和甲胎蛋白（AFP）在原髮性肝癌（PHC）診斷中的臨床意義。方法採用化學比色速率法、酶聯免疫吸附試驗和直接化學髮光法測定32例PHC患者、42例肝良性疾病患者、50例健康對照者血清AFU、GP73和AFP水平，併進行比較。結果 PHC組患者血清AFU、GP73、AFP水平[（789．4±475．2）mol/L、（205．5±50．8）μg/L、（977．3±637．4）ng/mL]高于肝良性疾病組[（378．3±127．9）mol/L、（77．5±28．9）μg/L、（6．09±1．57）ng/mL]和健康對照組[（347．8±119．6）mol/L、（33．5±7．5）μg/L、（4．17±1．26）ng/mL]，差異有統計學意義（P＜0．01）。以血清AFU、GP73、AFP水平為指標，檢測PHC患者的敏感性、特異性分彆為75．1％、73．4％、62．2％；86．8％、67．5％、97．8％，3項聯閤檢測的敏感性、特異性分彆為93．4％、85．4％。結論3項指標聯閤檢測，可顯著增加暘性檢齣率，有助于PHC的診斷、病情變化判彆及治療效果觀察。
목적：탐토혈청a-l-암조당감매（AFU）、고이기체당단백73（GP73）화갑태단백（AFP）재원발성간암（PHC）진단중적림상의의。방법채용화학비색속솔법、매련면역흡부시험화직접화학발광법측정32례PHC환자、42례간량성질병환자、50례건강대조자혈청AFU、GP73화AFP수평，병진행비교。결과 PHC조환자혈청AFU、GP73、AFP수평[（789．4±475．2）mol/L、（205．5±50．8）μg/L、（977．3±637．4）ng/mL]고우간량성질병조[（378．3±127．9）mol/L、（77．5±28．9）μg/L、（6．09±1．57）ng/mL]화건강대조조[（347．8±119．6）mol/L、（33．5±7．5）μg/L、（4．17±1．26）ng/mL]，차이유통계학의의（P＜0．01）。이혈청AFU、GP73、AFP수평위지표，검측PHC환자적민감성、특이성분별위75．1％、73．4％、62．2％；86．8％、67．5％、97．8％，3항연합검측적민감성、특이성분별위93．4％、85．4％。결론3항지표연합검측，가현저증가양성검출솔，유조우PHC적진단、병정변화판별급치료효과관찰。
Objective To investigate serum a-l-glycosidase(AFU) ,golgi glycoprotein 73(GP73) and alpha-fe-toprotein(AFP) in the diagnosis of primary hepatic carcinoma (PHC) clinical significance .Methods The rate of chemical colorimetric method ,method of enzyme-linked immune adsorbent (ELISA ) and direct chemiluminescence method were used to detect serum AFU ,GP73 and AFP level in 38 patients with PHC ,42 patients with benign liver diseases and 50 cases in normal control group ,then compared the datas in the three groups .Results Serum AFU , GP73 ,AFP level of PHC patients[(789 .4 ± 475 .2) mol/L ,(205 .5 ± 50 .8)μg/L ,(977 .3 ± 637 .4)ng/mL] ,were higher than those in the liver benign disease group(378 .3 ± 127 .9)mol/L ,(77 .5 ± 28 .9)μg/L ,(6 .09 ± 1 .57)ng/mL and the normal control group [(347 .8 ± 119 .6) mol/L ,(33 .5 ± 7 .5)μg/L ,(4 .17 ± 1 .26)ng/mL] ,the difference was statistically significant(P< 0 .01) .The sensitivity and specificity of serum AFU ,GP73 ,AFP levels as indicators to detect PHC were 75 .1% ,73 .4% ,62 .2% ;86 .8% ,67 .5% ,97 .8% ,the sensitivity and specificity of the united three test were 93 .4% ,85 .4% respectively .Conclusion The united three test can increase the rate of positive detection , which is beneficial to diagnosis of PHC ,discriminate course of the disease ,and observte the effect of treatment .
