CAJ | 학술논문

以荔浦芋无菌试管苗为材料，研究了试管苗保存过程中不同浓度多效唑（PP333）对试管苗保存效果的影响。研究结果表明，多效唑对芋种质资源的离体保存有显著影响，在光照时间14~16 h/d，光照强度1500 lx，室温（26±2）℃的培养条件下，在 MS+6-BA 2.5 mg/L+NAA 0.02 mg/L＋30 g/L 蔗糖的培养基中添加0.6 mg/L PP333，试管苗可保存240 d，存活率为89.7%，且恢复生长后试管苗形态正常、长势良好，与对照株无明显差异。
이려포우무균시관묘위재료，연구료시관묘보존과정중불동농도다효서（PP333）대시관묘보존효과적영향。연구결과표명，다효서대우충질자원적리체보존유현저영향，재광조시간14~16 h/d，광조강도1500 lx，실온（26±2）℃적배양조건하，재 MS+6-BA 2.5 mg/L+NAA 0.02 mg/L＋30 g/L 자당적배양기중첨가0.6 mg/L PP333，시관묘가보존240 d，존활솔위89.7%，차회복생장후시관묘형태정상、장세량호，여대조주무명현차이。
In this research, we studied the conservation effects of paclobutrazol with different concentrations on taro (Colocasia esculenta L. Schott) seedlings in vitro. The results showed that, paclobutrazol affected the in vitro conservation of taro germplasm resources significantly, and the best culture medium was MS+2.5 mg/L 6-BA+0.02 mg/L NAA+30 g/L sucrose+0.6 mg/L PP333, which could conserve plantlets for 240 days with the survival rate of 89.7%, under the cultural condition with illumination time of 14-16 hours per day, illumination intensity of 1 500 lx and room temperature of (26±2)℃. Furthermore, the recovered plantlets grew well and showed no difference in morphology with those of control treatment.