CAJ | 학술논문

采用单细胞培养法和组织块培养法从原代培养人胎儿成纤维细胞，并在不同体积分数胎牛血清（FBS）（0％、4％、8％、10％、12％）的条件下观察人胎儿成纤维细胞培养的状况并采用台盼蓝染色法进行活细胞计数．结果表明，早期由组织块培养法原代培养获取的细胞形态要比单细胞培养法原代培养获取的细胞稍好些，且在细胞传代后极少出现无法贴壁的死细胞．FBS对hFFs生长影响比较明显．在实际应用中，在体积分数8％FBS下，采用组织块培养法进行人胎儿成纤维细胞体外培养，效果良好．
채용단세포배양법화조직괴배양법종원대배양인태인성섬유세포，병재불동체적분수태우혈청（FBS）（0％、4％、8％、10％、12％）적조건하관찰인태인성섬유세포배양적상황병채용태반람염색법진행활세포계수．결과표명，조기유조직괴배양법원대배양획취적세포형태요비단세포배양법원대배양획취적세포초호사，차재세포전대후겁소출현무법첩벽적사세포．FBS대hFFs생장영향비교명현．재실제응용중，재체적분수8％FBS하，채용조직괴배양법진행인태인성섬유세포체외배양，효과량호．
The effect of single cell culture method and tissue block culture method on the growth of human fetal fi -broblasts cultured in vitro was compared ,and the best effect of which concentration of FBS on the growth of human fetal fibroblast cultured in vitro was studied .Human fetal fibroblasts were primarily cultured by single cell culture method and tissue block culture method,and the condition of human fetal fibroblasts on bovine serum (FBS) (0%, 4%, 8%, 10%,12%) was observed .Then by means of the method of trypan blue staining , the viable cells were counted .The results showed that , in the primary passage , the morphology of cell by tissue block culture method is better than that of cell cultured by single cell method , and dead cells of unable to stick wall were seldom appeared after passage .FBS had significant influence on the growth of hFFs .In the real application , when the concentration of FBS was 8%, using tis-sue block culture method to cultivate human fetal fibroblasts had a good effect .