中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2013年
36期
45-47
,共3页
黏膜皮肤淋巴结综合征%淋巴细胞亚群
黏膜皮膚淋巴結綜閤徵%淋巴細胞亞群
점막피부림파결종합정%림파세포아군
Mucocutaneous lymph node syndrome%Lymphocyte subpopulation
目的：观察川崎病儿童急性期外周血淋巴细胞亚群的变化，以探讨儿童川崎病的发病机制。方法：选择在本院最终确诊为川崎病的219例患儿作回顾性分析，106例健康体检儿童作为对照组，应用流式细胞术检测川崎病患儿治疗前及健康对照组儿童外周血淋巴细胞亚群CD3+细胞、CD3+CD4+细胞、CD3+CD8+细胞、CD16+CD56+细胞、CD19+细胞的百分率以及CD4+/CD8+的比值。结果：与健康对照组相比，川崎病患儿外周血CD19+细胞的百分率、CD4+/CD8+比值均明显升高（P<0.01）、而CD3+CD8+细胞、CD16+CD56+细胞的百分率则明显下降（P<0.01），但CD3+CD4+细胞百分率并无明显改变（P=0.24）；以本院淋巴细胞亚群的正常参考值为基准，川崎病儿童组急性期CD19+细胞升高的比例极高，为90.9%（199/219），而在健康对照组中仅仅为10.4%（11/106），两组比较差异有统计学意义（P<0.01）。结论：川崎病儿童急性期存在显著的免疫学紊乱，CD19+细胞的升高、CD3+CD8+细胞和CD16+CD56+细胞的下降可能均参与了川崎病的发病，而且这些淋巴细胞亚群的检测在诊断川崎病时，不失为一个无创且较简便的基于免疫学角度去评价患儿疾病状态的方法。
目的：觀察川崎病兒童急性期外週血淋巴細胞亞群的變化，以探討兒童川崎病的髮病機製。方法：選擇在本院最終確診為川崎病的219例患兒作迴顧性分析，106例健康體檢兒童作為對照組，應用流式細胞術檢測川崎病患兒治療前及健康對照組兒童外週血淋巴細胞亞群CD3+細胞、CD3+CD4+細胞、CD3+CD8+細胞、CD16+CD56+細胞、CD19+細胞的百分率以及CD4+/CD8+的比值。結果：與健康對照組相比，川崎病患兒外週血CD19+細胞的百分率、CD4+/CD8+比值均明顯升高（P<0.01）、而CD3+CD8+細胞、CD16+CD56+細胞的百分率則明顯下降（P<0.01），但CD3+CD4+細胞百分率併無明顯改變（P=0.24）；以本院淋巴細胞亞群的正常參攷值為基準，川崎病兒童組急性期CD19+細胞升高的比例極高，為90.9%（199/219），而在健康對照組中僅僅為10.4%（11/106），兩組比較差異有統計學意義（P<0.01）。結論：川崎病兒童急性期存在顯著的免疫學紊亂，CD19+細胞的升高、CD3+CD8+細胞和CD16+CD56+細胞的下降可能均參與瞭川崎病的髮病，而且這些淋巴細胞亞群的檢測在診斷川崎病時，不失為一箇無創且較簡便的基于免疫學角度去評價患兒疾病狀態的方法。
목적：관찰천기병인동급성기외주혈림파세포아군적변화，이탐토인동천기병적발병궤제。방법：선택재본원최종학진위천기병적219례환인작회고성분석，106례건강체검인동작위대조조，응용류식세포술검측천기병환인치료전급건강대조조인동외주혈림파세포아군CD3+세포、CD3+CD4+세포、CD3+CD8+세포、CD16+CD56+세포、CD19+세포적백분솔이급CD4+/CD8+적비치。결과：여건강대조조상비，천기병환인외주혈CD19+세포적백분솔、CD4+/CD8+비치균명현승고（P<0.01）、이CD3+CD8+세포、CD16+CD56+세포적백분솔칙명현하강（P<0.01），단CD3+CD4+세포백분솔병무명현개변（P=0.24）；이본원림파세포아군적정상삼고치위기준，천기병인동조급성기CD19+세포승고적비례겁고，위90.9%（199/219），이재건강대조조중부부위10.4%（11/106），량조비교차이유통계학의의（P<0.01）。결론：천기병인동급성기존재현저적면역학문란，CD19+세포적승고、CD3+CD8+세포화CD16+CD56+세포적하강가능균삼여료천기병적발병，이차저사림파세포아군적검측재진단천기병시，불실위일개무창차교간편적기우면역학각도거평개환인질병상태적방법。
Objective:To investigate the change and significance of lymphocyte subpopulation in the children with kawasaki disease(KD) during acute phase. Method:Two hundred and nineteen patients diagnosed as KD in the hospital were analyzed retrospectively,106 healthy children were chosen as control. Flow cytometric analysis was employed to detect the percentage of lymphocyte subpopulation(including CD3+cells、CD3+CD4+cells、CD3+CD8+cells、CD16+CD56+cells、CD19+cells)and CD4+/CD8+ratio in the KD patients before treatment and in the healthy children. Result:Compared with healthy control,the percentage of CD19+cells、and CD4+/CD8+ratio in patients with KD increased significantly(P<0.01),and the percentage of CD3+CD8+cells、CD16+CD56+cells decreased significantly(P<0.01),but there were not any significant change about the percentage of CD3+CD4+cells(P=0.24). Based on the standard value of lymphocyte subpopulation in the hospital,there were 199 KD patients who had increased CD19+cells percentage ,but only 11 children out of the healthy group had higher CD19+cells percentage(P<0.01). Conclusion:There are evident immunity disturbances during the acute phase of KD,these changes including decreased percentage of CD3+CD8+cells,CD16+CD56+cells and elevated CD19+cells percentage might take some role in the KD development,and detecting the percentage of lymphocyte subpopulation maybe helpful in the diagnosis of KD from one immunological view.
