CAJ | 학술논문

以“禾阳19”杨组培苗茎段获得的胚性愈伤组织为辐射诱变材料，进行了γ射线（60Co）辐射诱变、培养基上定向筛选杨抗病变异体的方法，结果显示，以茎段的胚性愈伤诱导率最高，达到100%；愈伤分化培养45 h胚性细胞分化，73~95 h胚性细胞大量形成，γ射线辐射处理为最佳时期；不同辐射剂量比较，30 Gy较接近半致死剂量；辐射后的胚性愈伤在培养基上定向筛选抗杨病变异体，得到生根试管苗408株；经大棚、大田连续进一步筛选获得1个抗杨白粉病的速生优系，定名为“禾抗3号”杨，连续3a抗病性检测结果表明，“禾抗3号”杨明显高于亲本和对照的抗病性，诱变性状稳定。
이“화양19”양조배묘경단획득적배성유상조직위복사유변재료，진행료γ사선（60Co）복사유변、배양기상정향사선양항병변이체적방법，결과현시，이경단적배성유상유도솔최고，체도100%；유상분화배양45 h배성세포분화，73~95 h배성세포대량형성，γ사선복사처리위최가시기；불동복사제량비교，30 Gy교접근반치사제량；복사후적배성유상재배양기상정향사선항양병변이체，득도생근시관묘408주；경대붕、대전련속진일보사선획득1개항양백분병적속생우계，정명위“화항3호”양，련속3a항병성검측결과표명，“화항3호”양명현고우친본화대조적항병성，유변성상은정。
In this study , the radiation mutagenic material being used is the embryonic stem callus cultured in“He Yang 19”Yang Plantlets , and the method of directed screening of resistant variants by the use ofγ-rays (60Co) irradiation is applied .The results showed the induction rate of stem embryogenic callus was highest(100%).The embryogenic cells differentiated 45 hours after the callus differentiation culture and lots of embryogenic cells formed 73~95 hours after the culture, which was the optimum time for γ irradiation treatment. Through comparative analysis of different doses, 30 Gy was found close to half-lethal dose. After directional screen of resistant variants from irradiated embryogenic callus in medium 408 rooting tube plantlets were obtained. A Powdery mildew resistant and fast growing best line named"He Yang resistance 3"was get from the further screen in the green house and field. The results of 3 years’ resistance examination showed that the resistance of " He Yang resistance 3 " was obviously higher than that of the parents and control and the mutation characters were stable.