CAJ | 학술논문

通过水稻连作对江苏地区稻田土进行不同的处理大田实验，探讨3种耕作方式对江苏地区稻田土细菌微生物多样性产生的影响。本实验采用Illumina miseq高通测序技术对细菌的16 S保守区进行了测序，研究了轮作、连作1年、连作3年3种状态下，稻田土细菌微生物的变化。结果显示：轮作的土壤环境微生物处理丰富度（Chao1和Ace）远高于连作，连作1年和3年的土壤，在细菌门和属水平的群落结构分析显示，连作后的土壤中放线菌的比例显著下降。
통과수도련작대강소지구도전토진행불동적처리대전실험，탐토3충경작방식대강소지구도전토세균미생물다양성산생적영향。본실험채용Illumina miseq고통측서기술대세균적16 S보수구진행료측서，연구료륜작、련작1년、련작3년3충상태하，도전토세균미생물적변화。결과현시：륜작적토양배경미생물처리봉부도（Chao1화Ace）원고우련작，련작1년화3년적토양，재세균문화속수평적군락결구분석현시，련작후적토양중방선균적비례현저하강。
In order to study the influence of 3 kinds of tillage to paddy soil microbial diversity, we conducted a field experiment in Jiangsu area. This experiment adopts the Illumina miseq sequencing of bacterial 16S conserved regions were sequenced. Study of the rotation, continuous cropping for one year, continuous cropping for three years, 3 kinds of bacteria in paddy soil conditions. The results showed that soil microbial of rotation treatment richness (Chao1 and Ace) is much higher than that of continuous cropping, cropping a year and three years of soil, analysis showed that the community structure in the bacterial phylum and genus levels, after continuous cropping soil actinomycetes ratio decreased significantly.