CAJ | 학술논문

在低温处理仔虾全长 cDNA 文库的筛选测序中，获得凡纳滨对虾(Litopenaeus vannmei)金属硫蛋白基因全长cDNA序列，该序列含有425个碱基，包含177 bp开放阅读框，上游98 bp的非编码区及下游150 bp的非编码区，编码58个氨基酸，其中半胱氨酸含量丰富，富含金属硫蛋白典型的Cys-X(1-3)-Cys 结构。多序列比对表明，凡纳滨对虾 MT 蛋白序列与美洲螯龙虾(Homarus americanus)MT 蛋白序列具最高同源性72.4%。Real-time PCR结果表明，凡纳滨对虾MT基因在卵巢组织中呈优势表达，在不同发育期的卵巢中的表达量都很高，在低温处理凡纳滨对虾肝胰腺组织中上调表达。实验所得结果为研究凡纳滨对虾金属硫蛋白基因在生殖发育和低温应激中的功能提供了参考。
재저온처리자하전장 cDNA 문고적사선측서중，획득범납빈대하(Litopenaeus vannmei)금속류단백기인전장cDNA서렬，해서렬함유425개감기，포함177 bp개방열독광，상유98 bp적비편마구급하유150 bp적비편마구，편마58개안기산，기중반광안산함량봉부，부함금속류단백전형적Cys-X(1-3)-Cys 결구。다서렬비대표명，범납빈대하 MT 단백서렬여미주오룡하(Homarus americanus)MT 단백서렬구최고동원성72.4%。Real-time PCR결과표명，범납빈대하MT기인재란소조직중정우세표체，재불동발육기적란소중적표체량도흔고，재저온처리범납빈대하간이선조직중상조표체。실험소득결과위연구범납빈대하금속류단백기인재생식발육화저온응격중적공능제공료삼고。
A 425 bp Litopenaeus vannmei Metallothionein gene (LvMT) cDNA sequence was got from the SMART cDNA library of low temperature treated young Litopenaeus vannmei. The cDNA contained a 98 bp 5′ terminal un-translated region (UTR), a 150 bp 3′terminal UTR and a 177 bp open reading frame (ORF) sequence that encoded 58 amino acids (AA). The deduced amino acids sequence was rich in cysteine and Cys-X(1-3)-Cys structure which was classic structure in MT genes. Multiple alignment showed high identity of MT gene from different species, and the iden-tity of Litopenaeus vannmei and Homarus americanus MT was up to 72.4%. The realtime PCR analysis revealed that LvMT mRNA mainly expressed in ovary and high level expression was found in different ovary development stages. Furthermore, the expression of LvMT mRNA was up-regulated in the low temperature treated Litopenaeus vannmei hepatopancreas. The data is useful for the function analysis of LvMT in ovary development and low temperature stress.