CAJ | 학술논문

利用磁珠富集法,以生物素标记的(CA)15为探针,构建了蛇鳄龟(Chelydra serpentina L.)微卫星富集文库。通过PCR法从富集文库中共筛选出70条微卫星序列,一共设计了48对微卫星引物,采用PCR扩增的方法从中筛选出36对引物,对一个蛇鳄龟养殖群体进行遗传多样性分析。通过分析,36个位点获得的等位基因数从2-9不等,平均为4.361,有效等位基因为1.461-7.767,平均为3.498。等位基因片段大小为56-342 bp,观测杂合度为0.067-1.000,平均为0.725；期望杂合度为0.316-0.850,平均0.600；多态信息含量为0.2655-0.8359,平均为0.5573；结果表明此蛇鳄龟养殖群体存在较高的遗传多样性水平。群体内固定系数?0.688-0.856,平均为?0.214,说明蛇鳄龟群体中杂合子过剩。
이용자주부집법,이생물소표기적(CA)15위탐침,구건료사악구(Chelydra serpentina L.)미위성부집문고。통과PCR법종부집문고중공사선출70조미위성서렬,일공설계료48대미위성인물,채용PCR확증적방법종중사선출36대인물,대일개사악구양식군체진행유전다양성분석。통과분석,36개위점획득적등위기인수종2-9불등,평균위4.361,유효등위기인위1.461-7.767,평균위3.498。등위기인편단대소위56-342 bp,관측잡합도위0.067-1.000,평균위0.725；기망잡합도위0.316-0.850,평균0.600；다태신식함량위0.2655-0.8359,평균위0.5573；결과표명차사악구양식군체존재교고적유전다양성수평。군체내고정계수?0.688-0.856,평균위?0.214,설명사악구군체중잡합자과잉。
To isolate and identify polymorphic microsatellite markers which would be used for evaluating the genetic diversity of Chelydra serpentina L. An enriched microsatellite library of C. serpentina was constructed by the mag-netic-bead enrichment method used biotinylated (CA)15 probe. Screened the enriched microsatellite library by PCR am-plification technique, seventy microsatellite sequences were obtained. To analyze genetic diversity of a cultured C. ser-pentina, forty eight pairs of primers were designed on the basis of the microsatellite sequences and 36 of that were screened by PCR amplification technique. In these microsatellite loci, the number of alleles per locus ranged from two to nine with an average of 4.361. The number of effective allele ranged from 1.461 to 7.767 with an average of 3.498. The size of allele fragment ranged between 56 and 342 bp. The observed heterozygosity varied from 0.067 to 1.000 with an average of 0.725. The expected heterozygosity varied from 0.316 to 0.850 with an average of 0.600. Polymorphism information content (PIC) ranged from 0.2655 to 0.8359 with an average of 0.5573. The average fixation index coeffi-cient of population was ?0.214, which suggested surplus heterozygotes in the population. These results indicated that the cultured C. serpentina populations have relatively high genetic diversity.