CAJ | 학술논문

以肉食性南方大口鲇(Silurus soldatovi meridonalis Chen)为对象，研究采用PCR-DGGE指纹技术对其仔稚鱼胃肠道分化过程及细菌群落结构进行了对比分析，进而探讨了细菌群落与胃肠道分化的关系。消化道外部形态和胃肠切片显示13日龄样品起，胃肠在结构上出现较大的分化。DGGE分析结果显示胃肠道细菌群落的分化始于18日龄：从18日龄样品起，肠内细菌群落明显开始分化，但在随后的18-33日龄期间细菌群落结构相对稳定，而胃内细菌群落则一直处于动态变化中。这说明，南方大口鲇仔稚鱼的个体发育过程中，随着胃肠在组织结构上的分化，其消化道细菌群落结构也出现了明显分化，并且胃和肠内细菌群落结构的分化在时间上存在差异。该研究结果将为深入研究胃和肠内细菌群落的功能差异提供基础资料。
이육식성남방대구점(Silurus soldatovi meridonalis Chen)위대상，연구채용PCR-DGGE지문기술대기자치어위장도분화과정급세균군락결구진행료대비분석，진이탐토료세균군락여위장도분화적관계。소화도외부형태화위장절편현시13일령양품기，위장재결구상출현교대적분화。DGGE분석결과현시위장도세균군락적분화시우18일령：종18일령양품기，장내세균군락명현개시분화，단재수후적18-33일령기간세균군락결구상대은정，이위내세균군락칙일직처우동태변화중。저설명，남방대구점자치어적개체발육과정중，수착위장재조직결구상적분화，기소화도세균군락결구야출현료명현분화，병차위화장내세균군락결구적분화재시간상존재차이。해연구결과장위심입연구위화장내세균군락적공능차이제공기출자료。
To explore the potential relationship between differentiation of gastrointestinal tract and the gastrointestinal microbiota colonization, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was applied to analyze the gastrointestinal microbiota alteration of Silurus soldatovi meridonalis Chen with the differentiation of gas-trointestinal tract. The differentiation of the intestinal microbiota started from 18-day samples and kept relatively stable in the following 23-, 28- and 33-day samples. However, the differentiation of the stomach microbiota was in dynamic variation process during the experiment. Morphological observation and histological slices of the gastrointestinal tract suggested a significant differentiation started at the developmental stage of 13-day. It indicated that with the differen-tiation of digestive system, the differentiations of bacteria community structures of both stomach and gut were different in spatiotempord.