CAJ | 학술논문

建立环介导等温扩增技术快速检测铜绿假单胞菌的方法。以铜绿假单胞菌特异性基因ETA为靶基因，设计特异性引物，同时对扩增条件进行优化，以41株铜绿假单胞菌测定该方法的灵敏度，并以15株其他菌株对环介导等温扩增技术方法的特异性进行检验。结果显示，建立的环介导等温扩增方法特异性好，灵敏度达到2.2 pg DNA/管。
건립배개도등온확증기술쾌속검측동록가단포균적방법。이동록가단포균특이성기인ETA위파기인，설계특이성인물，동시대확증조건진행우화，이41주동록가단포균측정해방법적령민도，병이15주기타균주대배개도등온확증기술방법적특이성진행검험。결과현시，건립적배개도등온확증방법특이성호，령민도체도2.2 pg DNA/관。
To establish a method for determination of Pseudomonas aeruginosa by using loop-mediated isothermal amplification (LAMP) technology. The LAMP primers were designed on the basis of ETA gene in Pseudomonas aeruginosa. The LAMP reaction mixture was optimized. The detection was evaluated by 41 strains of Pseudomonas aeruginosa and 15 other strains. The determination limit of LAMP when testing Pseudomonas aeruginosa templates was 2.2 pg DNA/tube.