实用医学杂志
實用醫學雜誌
실용의학잡지
THE JOURNAL OF PRACTICAL MEDICINE
2011年
13期
2454-2456
,共3页
保存温度%保存时间%HCV-RNA%荧光定量PCR
保存溫度%保存時間%HCV-RNA%熒光定量PCR
보존온도%보존시간%HCV-RNA%형광정량PCR
目的:探索不同保存温度和保存时间对血清HCV-RNA荧光定量检测结果的影响.方法:收集HCV阳性血清标本16份并平均分成6份分别置于室温、4℃和-20℃保存1 d和1周后用荧光定量PCR法检测结果,利用SPSS13.0软件对数据进行处理.结果:HCV阳性患者血清在室温(25 ± 2)℃、4℃和-20℃保存1 d,各组荧光定量结果无统计学差异(F = 0.012,0.447,0.230;P = 0.913,0.507,0.796);保存1周,各组结果无统计学差异(F = 0.059,0.308,0.184;P = 0.808,0.582,0.833);根据温度分组后血清保存1 d和保存1周结果无统计学差异(F = 0.055,0.000,0.103;P = 0.816,0.990,0.751).结论:血清在室温、4℃和-20℃条件下保存1周对HCV-RNA的荧光定量检测结果无显著影响.
目的:探索不同保存溫度和保存時間對血清HCV-RNA熒光定量檢測結果的影響.方法:收集HCV暘性血清標本16份併平均分成6份分彆置于室溫、4℃和-20℃保存1 d和1週後用熒光定量PCR法檢測結果,利用SPSS13.0軟件對數據進行處理.結果:HCV暘性患者血清在室溫(25 ± 2)℃、4℃和-20℃保存1 d,各組熒光定量結果無統計學差異(F = 0.012,0.447,0.230;P = 0.913,0.507,0.796);保存1週,各組結果無統計學差異(F = 0.059,0.308,0.184;P = 0.808,0.582,0.833);根據溫度分組後血清保存1 d和保存1週結果無統計學差異(F = 0.055,0.000,0.103;P = 0.816,0.990,0.751).結論:血清在室溫、4℃和-20℃條件下保存1週對HCV-RNA的熒光定量檢測結果無顯著影響.
목적:탐색불동보존온도화보존시간대혈청HCV-RNA형광정량검측결과적영향.방법:수집HCV양성혈청표본16빈병평균분성6빈분별치우실온、4℃화-20℃보존1 d화1주후용형광정량PCR법검측결과,이용SPSS13.0연건대수거진행처리.결과:HCV양성환자혈청재실온(25 ± 2)℃、4℃화-20℃보존1 d,각조형광정량결과무통계학차이(F = 0.012,0.447,0.230;P = 0.913,0.507,0.796);보존1주,각조결과무통계학차이(F = 0.059,0.308,0.184;P = 0.808,0.582,0.833);근거온도분조후혈청보존1 d화보존1주결과무통계학차이(F = 0.055,0.000,0.103;P = 0.816,0.990,0.751).결론:혈청재실온、4℃화-20℃조건하보존1주대HCV-RNA적형광정량검측결과무현저영향.