中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
4期
482-485
,共4页
朱丹丹%张从雨%汪东树%刘刚
硃丹丹%張從雨%汪東樹%劉剛
주단단%장종우%왕동수%류강
1-磷脂酰肌醇3-激酶%信号传导%白细胞介素8%趋化因子,CXC%肝肿瘤/病理学%肿瘤转移
1-燐脂酰肌醇3-激酶%信號傳導%白細胞介素8%趨化因子,CXC%肝腫瘤/病理學%腫瘤轉移
1-린지선기순3-격매%신호전도%백세포개소8%추화인자,CXC%간종류/병이학%종류전이
1-phosphatidylinositol 3-kinase%Signal transduction%Interleukin-8%Chemokines,CXC%Liver neoplasms/pathology%Neoplasm metastasis
目的 探讨磷酸肌醇3激酶(PI3K)信号通路在白细胞介素8(IL-8)/细胞表面G-蛋白偶联受体趋化因子受体(CXCR2)介导的肝癌生长、转移过程中的作用.方法 收集肝癌组织、血清标本82例,良性组织及血清标本21例,用ELISA法检测各组血清中IL-8的水平;用免疫组织化学法检测CXCR2的表达情况,并对其与各临床病理参数进行相关性分析.选取不同分期的肝癌新鲜组织,用Western blot法检测CXCR2、磷酸肌醇3激酶γ(PI3Kγ)、丝氨酸/苏氨酸蛋白激酶(AKT)、核因子-κB(NF-κB)的表达情况,分析各蛋白质表达的关系.结果 Ⅰ、Ⅱ、Ⅲ、Ⅳ期肝癌患者血清中IL-8的表达水平依次为(120.34±15.52) pg/ml、(221.60±23.10) pg/ml、(279.50±24.25) pg/ml、(324.81±22.50) pg/ml,随着肝癌恶性程度的升高,IL-8的水平不断增加,尤以肝癌Ⅳ期最为显著;CXCR2的表达与肝癌的病理学分期、分化程度、淋巴结转移和远处转移有关(P＜0.05);肝癌组织中CX-CR2的表达与血清中IL-8的水平具有一致性,且与PI3 Kγ、AKT、NF-κB蛋白质的活化程度有关.结论 IL-8/CXCR2能够介导肝癌细胞的生长、转移,其机制可能与PI3K信号通路的激活有关.
目的 探討燐痠肌醇3激酶(PI3K)信號通路在白細胞介素8(IL-8)/細胞錶麵G-蛋白偶聯受體趨化因子受體(CXCR2)介導的肝癌生長、轉移過程中的作用.方法 收集肝癌組織、血清標本82例,良性組織及血清標本21例,用ELISA法檢測各組血清中IL-8的水平;用免疫組織化學法檢測CXCR2的錶達情況,併對其與各臨床病理參數進行相關性分析.選取不同分期的肝癌新鮮組織,用Western blot法檢測CXCR2、燐痠肌醇3激酶γ(PI3Kγ)、絲氨痠/囌氨痠蛋白激酶(AKT)、覈因子-κB(NF-κB)的錶達情況,分析各蛋白質錶達的關繫.結果 Ⅰ、Ⅱ、Ⅲ、Ⅳ期肝癌患者血清中IL-8的錶達水平依次為(120.34±15.52) pg/ml、(221.60±23.10) pg/ml、(279.50±24.25) pg/ml、(324.81±22.50) pg/ml,隨著肝癌噁性程度的升高,IL-8的水平不斷增加,尤以肝癌Ⅳ期最為顯著;CXCR2的錶達與肝癌的病理學分期、分化程度、淋巴結轉移和遠處轉移有關(P＜0.05);肝癌組織中CX-CR2的錶達與血清中IL-8的水平具有一緻性,且與PI3 Kγ、AKT、NF-κB蛋白質的活化程度有關.結論 IL-8/CXCR2能夠介導肝癌細胞的生長、轉移,其機製可能與PI3K信號通路的激活有關.
목적 탐토린산기순3격매(PI3K)신호통로재백세포개소8(IL-8)/세포표면G-단백우련수체추화인자수체(CXCR2)개도적간암생장、전이과정중적작용.방법 수집간암조직、혈청표본82례,량성조직급혈청표본21례,용ELISA법검측각조혈청중IL-8적수평;용면역조직화학법검측CXCR2적표체정황,병대기여각림상병리삼수진행상관성분석.선취불동분기적간암신선조직,용Western blot법검측CXCR2、린산기순3격매γ(PI3Kγ)、사안산/소안산단백격매(AKT)、핵인자-κB(NF-κB)적표체정황,분석각단백질표체적관계.결과 Ⅰ、Ⅱ、Ⅲ、Ⅳ기간암환자혈청중IL-8적표체수평의차위(120.34±15.52) pg/ml、(221.60±23.10) pg/ml、(279.50±24.25) pg/ml、(324.81±22.50) pg/ml,수착간암악성정도적승고,IL-8적수평불단증가,우이간암Ⅳ기최위현저;CXCR2적표체여간암적병이학분기、분화정도、림파결전이화원처전이유관(P＜0.05);간암조직중CX-CR2적표체여혈청중IL-8적수평구유일치성,차여PI3 Kγ、AKT、NF-κB단백질적활화정도유관.결론 IL-8/CXCR2능구개도간암세포적생장、전이,기궤제가능여PI3K신호통로적격활유관.
Objective To explore the role of phosphatidylinositol 3-kinase (PI3K) signal pathway in interleukin-8 (IL-8)/chemokine (C-X-C Motif) receptor 2 (CXCR2)-mediated growth and metastasis of liver cancer.Methods Eighty-two cases of liver cancer tissues and serum samples,and 21 cases of normal tissues and serum samples were collected.The level of serum IL-8 was measured by enzyme-linked immunesorbent assay (ELISA).Expression of CXCR2 was detected by immunohistochemical method,and its correlation with clinicopathological parameters was also analyzed.Different stages of liver cancer fresh tissues were selected,and expressions of CXCR2,PI3Kγ,AKT,and NF-κB were detected by Westeru blotting analyses.Results The levels of serum IL-8 in liver cancer patients (stages Ⅰ,Ⅱ,Ⅲ,Ⅳ) were (120.34 ±15.52) pg/ml,(221.60 ±23.10) pg/ml,(279.50 ±24.25) pg/ml,(324.81 ±22.50) pg/ml,respectively.With the increasing degree of liver cancers,levels of IL-8 were increased,most significant was in the Ⅳ period of liver cancer; the expression of CXCR2 was significantly correlated with pathological stages,degree of differentiation,lymph node and distant metastases of liver cancer (P ＜ 0.05) ; serum IL-8 levels were consistent with expression of CXCR2 in liver cancer tissues,which had a strong correlation with activation of PI3K γ,AKT,and NF-κ B proteins.Conclusions IL-8/CXCR2 was able to mediate the growth and metastasis of liver cancers,which might be through the activation of PI3K signaling pathway.
