CAJ | 학술논문

目的：探讨去甲斑蝥素对人直肠癌Colo 320细胞的诱导凋亡作用．方法采用不同浓度的去甲斑蝥素（noncantharidin，NCTD）作用于人直肠癌Colo 320细胞24 h，应用光镜和透射电镜观察细胞形态学和细胞超微结构的变化，流式细胞的方法检测细胞生长周期，Western blot法检测细胞Bag-1和Bcl-2蛋白表达．结果光镜及透射电镜观察可见人直肠癌Colo 320细胞出现典型的凋亡形态学和超微结构的改变．流式细胞仪分析显示：经5滋g/mL、10滋g/mL和20滋g/mL浓度NCTD处理人直肠癌Colo320细胞24 h后， G0/G1期和S期细胞逐渐减少， G2/M期细胞逐渐增多，呈剂量依赖关系．Western blot检测显示，Bcl-2、Bag-1蛋白的表达均下降．结论 NCTD对人直肠癌Colo 320细胞具有促进凋亡、干扰细胞的有丝分裂、将细胞阻滞于G2/M分裂期和抑制Bag-1及Bcl-2蛋白表达的作用．
목적：탐토거갑반모소대인직장암Colo 320세포적유도조망작용．방법채용불동농도적거갑반모소（noncantharidin，NCTD）작용우인직장암Colo 320세포24 h，응용광경화투사전경관찰세포형태학화세포초미결구적변화，류식세포적방법검측세포생장주기，Western blot법검측세포Bag-1화Bcl-2단백표체．결과광경급투사전경관찰가견인직장암Colo 320세포출현전형적조망형태학화초미결구적개변．류식세포의분석현시：경5자g/mL、10자g/mL화20자g/mL농도NCTD처리인직장암Colo320세포24 h후， G0/G1기화S기세포축점감소， G2/M기세포축점증다，정제량의뢰관계．Western blot검측현시，Bcl-2、Bag-1단백적표체균하강．결론 NCTD대인직장암Colo 320세포구유촉진조망、간우세포적유사분렬、장세포조체우G2/M분렬기화억제Bag-1급Bcl-2단백표체적작용．
Objective To investigate the effect of norcantharidin on growth inhibition and induction of apoptosis of human rectal cancer Colo 320 cells. Methods Norcantharidin (NCTD) in different concentrations were added to rectal cancer Colo 320 cells. Morphological characteristics of apoptosis were observed using the light microscope and transmission electron microscope. The expressions of Bag-1 and Bcl-2 proteins were tested by Western blotting. The growth inhibition of Colo 320 cells on the cell cycle was observed by flow cytometry. Results The apoptosis morphological changes of Colo 320 cells were observed by the light microscope and transmission electron microscopy. Flow cytometry analysis showed that the cell count of G2/M phase in experimental group was higher than that in control group ( <0.05) but the cell counts of G0/G1 and S phases have decreased in experimental group after treatment with NCTD at the concentrations of 5μg/mL, 10μg/mL and 20 μg/mL, and presented dosage dependence relations. The expressions of Bag-1 and Bcl-2 proteins have decreased. Conclusion Norcantharidin has inhibitory effect on rectal cancer Colo 320 cells, and the effect may be related to the cell cycle arrest and apoptosis.