云南化工
雲南化工
운남화공
YUNNAN CHEMICAL TECHNOLOGY
2012年
5期
45-47
,共3页
高效液相色谱法(HPLC)%纽甜%白酒。
高效液相色譜法(HPLC)%紐甜%白酒。
고효액상색보법(HPLC)%뉴첨%백주。
High Performance Liquid Chromatography (HPLC)%Neotame%distilled spirit
建立了一种高效液相色谱法(HPLC)测定白酒中纽甜含量的方法。沸水浴加热除去乙醇,纯水定容至相应体积,采用二极管阵列检测器(DAD),AgilentEclipseXDB-C18柱(4.6mm×250mm,5um),流动相为乙腈·离子对缓冲溶液(体积比25:75),进样量20uL,柱温30℃,检测波长218nm进行检测,外标法定量。添加回收率范围为97%-102%。
建立瞭一種高效液相色譜法(HPLC)測定白酒中紐甜含量的方法。沸水浴加熱除去乙醇,純水定容至相應體積,採用二極管陣列檢測器(DAD),AgilentEclipseXDB-C18柱(4.6mm×250mm,5um),流動相為乙腈·離子對緩遲溶液(體積比25:75),進樣量20uL,柱溫30℃,檢測波長218nm進行檢測,外標法定量。添加迴收率範圍為97%-102%。
건립료일충고효액상색보법(HPLC)측정백주중뉴첨함량적방법。비수욕가열제거을순,순수정용지상응체적,채용이겁관진렬검측기(DAD),AgilentEclipseXDB-C18주(4.6mm×250mm,5um),류동상위을정·리자대완충용액(체적비25:75),진양량20uL,주온30℃,검측파장218nm진행검측,외표법정량。첨가회수솔범위위97%-102%。
A method for determination of Neotame in Distilled Spirit by high performance liquid chromatography (HPLC) has been established. Ethanol is removed by heating with boiling water and constant volume to the corresponding volume with water. Neotame is determined by HPLC with the factors such as the detector DAD, the column of Agilent Eclipse XDB-C18 (4.6 mm × 250 mm, 5 um), mobile phase ratio (Vacetonitrile : Vion pair buffer solution = 25:75), sample size 20 uL, the temperature of the column at 30℃ and testing wavelength at 218 nm. It is quantified using external standard. The recoveries of the method with satisfactory precision ranged from 97% to 102%. This method is simple and suitable for the determination of Neotame in distilled Spirit.