CAJ | 학술논문

目的：研究大麻素CB2受体激动剂HU308对脂多糖（LPS）诱导小胶质细胞激活后NO及IL-6分泌的影响。方法体外培养小鼠小胶质细胞株（BV-2细胞），分为对照组、LPS刺激组及干预组（LPS+HU308）。通过显微镜观察各组小胶质细胞的形态学变化，CCK-8法检测各组小胶质细胞的增殖情况，Griess法检测各组NO含量，ELISA法检测各组IL-6水平。结果LPS刺激组的小胶质细胞中出现大量胞体增大，伪足粗短或消失的细胞和一些坏死细胞，细胞增殖较差，NO及IL-6表达水平显著增高。经大麻素CB2受体激动剂HU308干预后，大部分细胞胞体稍大，伪足尚明显，细胞破坏程度轻，增殖较好，炎性因子表达均明显下降。结论激动小胶质细胞表面的大麻素CB2受体，可以减轻LPS造成的小胶质细胞过度活化或损伤，抑制其炎性因子N0及IL-6的分泌，从而达到中枢神经系统炎性损伤后的神经保护作用。
목적：연구대마소CB2수체격동제HU308대지다당（LPS）유도소효질세포격활후NO급IL-6분비적영향。방법체외배양소서소효질세포주（BV-2세포），분위대조조、LPS자격조급간예조（LPS+HU308）。통과현미경관찰각조소효질세포적형태학변화，CCK-8법검측각조소효질세포적증식정황，Griess법검측각조NO함량，ELISA법검측각조IL-6수평。결과LPS자격조적소효질세포중출현대량포체증대，위족조단혹소실적세포화일사배사세포，세포증식교차，NO급IL-6표체수평현저증고。경대마소CB2수체격동제HU308간예후，대부분세포포체초대，위족상명현，세포파배정도경，증식교호，염성인자표체균명현하강。결론격동소효질세포표면적대마소CB2수체，가이감경LPS조성적소효질세포과도활화혹손상，억제기염성인자N0급IL-6적분비，종이체도중추신경계통염성손상후적신경보호작용。
Objective To study the effect in secretion of NO and IL-6 of cannabinoid CB2 receptor agonist (HU308) on microglia induced by lipopolysaccharide (LPS). Methods vitro culture BV-2 microglia were divided into control group, LPS stimulation group and intervention group (LPS + HU308). The morphological changes in microglia were observed by microscope, the proliferation in microglia was detected by CCK-8, the NO contents were detected by Griess reaction, the IL-6 contents were detected by ELISA. Results A large number of microglia in LPS stimulation group showed auxetic body, stubby or disappeared pseudopodium and necrotic cells, cell proliferation was poor, the expression level of NO and IL-6 increased significantly. After the intervention of CB2 receptor agonist (HU308), most of microglia showed slightly large body, obvious pseudopodia and light damage, cell proliferation was better, the expression of inflammatory factor decreased significantly. Conclusion Activating CB2 receptors expressing in microglia can alleviate the excessive activation of microglia caused by LPS, inhibit the secretion of N0 and IL-6, so as to achieve the protective effect of central nervous system inflammatory injury.