CAJ | 학술논문

采用HPLC的模式,分别研究温敏性色谱固定相对不同极性的类固醇、多肽等物质的驻留性能,考察物质极性等性质对其驻留性能的影响。实验发现,多肽样品随疏水性的增强,保留时间发生不同程度的延后现象,疏水性越强延后程度越明显。同时发现,溶液中的高离子强度可以增强蛋白质和多肽等生物大分子与疏水性固定相介质之间的疏水作用。
채용HPLC적모식,분별연구온민성색보고정상대불동겁성적류고순、다태등물질적주류성능,고찰물질겁성등성질대기주류성능적영향。실험발현,다태양품수소수성적증강,보류시간발생불동정도적연후현상,소수성월강연후정도월명현。동시발현,용액중적고리자강도가이증강단백질화다태등생물대분자여소수성고정상개질지간적소수작용。
Poly（N-isopropylacrylamide）（PNIPAAm） grafted to（aminopropyl）silica by an surface two-bond coupling was used as an HPLC packing material.The surface properties and functions of the stationary phase of some steroids and some peptides were controlled by external temperature.Isocratic elution by aqueous mobile phase of alone was the basis for separation of peptides.The elution behaviors of peptides with different hydrophobicity and isoelectric point were controlled by the temperature changes without organic solvents in the mobile phase.Separation was optimized just by changing the temperature,instead of changing the mobile phase composition.With increasing temperature,increased interaction between solutes and surfaces of the stationary phases was observed.