CAJ | 학술논문

为阐明鸡蛔虫湖南分离株线粒体烟酰胺腺嘌呤二核苷酸（NADH）脱氢酶亚单位4（nad4）基因部分序列（pnad4）遗传变异情况,利用聚合酶链式反应（PCR）扩增鸡蛔虫的pnad4,并对序列进行比对。结果表明：所获得的pnad4序列长度均为408 bp。由于鸡蛔虫pnad4序列种内相对保守,种间差异较大,故可作为种间遗传变异研究的标记,从而为鸡蛔虫的分类鉴定及进一步的分子流行病学调查奠定基础。
위천명계회충호남분리주선립체연선알선표령이핵감산（NADH）탈경매아단위4（nad4）기인부분서렬（pnad4）유전변이정황,이용취합매련식반응（PCR）확증계회충적pnad4,병대서렬진행비대。결과표명：소획득적pnad4서렬장도균위408 bp。유우계회충pnad4서렬충내상대보수,충간차이교대,고가작위충간유전변이연구적표기,종이위계회충적분류감정급진일보적분자류행병학조사전정기출。
For elaborating genetic variation,the subunit gene nad4 of NADH of Ascaridia galli isolated from Hunan Province was sequenced and analyzed.PCR amplification which amided at part of nad4 gene（pnad4）had been performed,and the amplicons had been compared to other sequences stored at GenBank.Results revealed that all the size of the amplicons were 408 bp.Due to the conservation of pnad4 gene was only in the same specie,it had been thought to be genetic marker.This study had laid the foundation for identification and molecular epidemiology investigation of Ascaridia galli.