仲恺农业工程学院学报
仲愷農業工程學院學報
중개농업공정학원학보
JOURNAL OF ZHONGKAI UNIVERSITY OF AGRICULTURE AND TECHNOLOGY
2012年
1期
44-47,55
,共5页
虎舌红(Ardisia%mamillata)%总黄酮%大孔树脂
虎舌紅(Ardisia%mamillata)%總黃酮%大孔樹脂
호설홍(Ardisia%mamillata)%총황동%대공수지
Ardisia mamillata%total flavonoid%macroporous resin
对大孔吸附树脂富集纯化虎舌红（Ardisia mamillata）总黄酮的最佳工艺参数进行了研究，并比较了4种大孔树脂对虎舌红总黄酮的吸附与解吸效果，从中筛选出适合分离纯化虎舌红总黄酮的树脂，同时对其吸附和解吸条件进行了探讨．结果表明，AB-8型为较理想树脂，其纯化总黄酮的最优工艺条件为：上样液质量浓度为0．34mg／mL，吸附流速为2．0mL／min，吸附pH值为5．0，洗脱剂为3BV（90mL）体积分数70％的乙醇，解吸流速为1．50mL／min．经AB-8型树脂纯化后，总黄酮纯度由原来的8．92％提高到67．79％，提高了7．6倍．
對大孔吸附樹脂富集純化虎舌紅（Ardisia mamillata）總黃酮的最佳工藝參數進行瞭研究，併比較瞭4種大孔樹脂對虎舌紅總黃酮的吸附與解吸效果，從中篩選齣適閤分離純化虎舌紅總黃酮的樹脂，同時對其吸附和解吸條件進行瞭探討．結果錶明，AB-8型為較理想樹脂，其純化總黃酮的最優工藝條件為：上樣液質量濃度為0．34mg／mL，吸附流速為2．0mL／min，吸附pH值為5．0，洗脫劑為3BV（90mL）體積分數70％的乙醇，解吸流速為1．50mL／min．經AB-8型樹脂純化後，總黃酮純度由原來的8．92％提高到67．79％，提高瞭7．6倍．
대대공흡부수지부집순화호설홍（Ardisia mamillata）총황동적최가공예삼수진행료연구，병비교료4충대공수지대호설홍총황동적흡부여해흡효과，종중사선출괄합분리순화호설홍총황동적수지，동시대기흡부화해흡조건진행료탐토．결과표명，AB-8형위교이상수지，기순화총황동적최우공예조건위：상양액질량농도위0．34mg／mL，흡부류속위2．0mL／min，흡부pH치위5．0，세탈제위3BV（90mL）체적분수70％적을순，해흡류속위1．50mL／min．경AB-8형수지순화후，총황동순도유원래적8．92％제고도67．79％，제고료7．6배．
The optimal conditions of purification of total flavonoids from Ardisia mamillata with macro- porous resin were studied, i.e. the absorption and desorption properties of four kinds of macroporous resin. Results showed that AB-8 macroporous resin had the best adsorption capacity under following technic condi- tions： the initial concentration, flow velouty, pH value and desovption flow velouty were at 0. 34 mg/mL, 2. 0 mL/min, 5.0 and 1.50 mL/min, respectively, with 3BV 70% ethanol as washiny solution. The purity of flavonoids was from 8.92% to 67.79%, which was 7.6 times higher than that of the original sample.
