CAJ | 학술논문

利用透明质酸在196nm波长紫外光下有吸收峰，并且透明质酸在0-1．0g／L的质量浓度范围内与吸光度有良好的线性关系，采用紫外光谱法测定了发酵法生产透明质酸发酵液中透明质酸的浓度．结果表明，发酵液中透明质酸质量浓度为0．98g／L，平均回收率为100．45％，相对标准偏差为0．78％，认为紫外光谱法是一种可准确测定透明质酸浓度的方法．
이용투명질산재196nm파장자외광하유흡수봉，병차투명질산재0-1．0g／L적질량농도범위내여흡광도유량호적선성관계，채용자외광보법측정료발효법생산투명질산발효액중투명질산적농도．결과표명，발효액중투명질산질량농도위0．98g／L，평균회수솔위100．45％，상대표준편차위0．78％，인위자외광보법시일충가준학측정투명질산농도적방법．
Hyluronic acid （HA） had absorption peak at 196 nm spectrophotometer, and the absorbance had good correlation with 0 - 1.0 g/L HA, so ultraviolet spectrophotomete was adopted to detect the con- centration of HA from fermentation broth. The result showed that the mass concentration of HA in the fer- mentation broth was 0. 98 g/L, the average recovery rate was 100. 45%, and relative standard deviation was 0. 78%. The result showed that ultraviolet spectrophotomete was an adaptable of accurate method for measuring the content of HA.