福建分析测试
福建分析測試
복건분석측시
FUJIAN ANALYSIS & TESTING
2012年
1期
29-33
,共5页
药残%硝基呋喃%液相色谱串联质谱
藥殘%硝基呋喃%液相色譜串聯質譜
약잔%초기부남%액상색보천련질보
Drugs residues%Nitrofuran%LC-MS-MS
硝基呋喃类药物及其代谢产物可使实验动物发生癌变、基因突变,同时具有生殖毒性,可能对人体产生危害。因此,硝基呋喃含量的检测在食品安全中是至关重要的,本文采用液相色谱串联质谱技术检测四种硝基呋喃类代谢物。硝基呋喃类代谢物在酸性条件下经过2-硝基苯甲醛衍生化,用乙酸乙酯提取纯化,电喷雾离子化,液相色谱-串联质谱检测。结果表明,四种硝基呋喃类药物的色谱峰面积与浓度在0.1～10.0 ng/mL范围内均呈现很好的线性关系,该方法具有操作简便、灵敏、准确等优点。
硝基呋喃類藥物及其代謝產物可使實驗動物髮生癌變、基因突變,同時具有生殖毒性,可能對人體產生危害。因此,硝基呋喃含量的檢測在食品安全中是至關重要的,本文採用液相色譜串聯質譜技術檢測四種硝基呋喃類代謝物。硝基呋喃類代謝物在痠性條件下經過2-硝基苯甲醛衍生化,用乙痠乙酯提取純化,電噴霧離子化,液相色譜-串聯質譜檢測。結果錶明,四種硝基呋喃類藥物的色譜峰麵積與濃度在0.1～10.0 ng/mL範圍內均呈現很好的線性關繫,該方法具有操作簡便、靈敏、準確等優點。
초기부남류약물급기대사산물가사실험동물발생암변、기인돌변,동시구유생식독성,가능대인체산생위해。인차,초기부남함량적검측재식품안전중시지관중요적,본문채용액상색보천련질보기술검측사충초기부남류대사물。초기부남류대사물재산성조건하경과2-초기분갑철연생화,용을산을지제취순화,전분무리자화,액상색보-천련질보검측。결과표명,사충초기부남류약물적색보봉면적여농도재0.1～10.0 ng/mL범위내균정현흔호적선성관계,해방법구유조작간편、령민、준학등우점。
Nitrofurans and their metabolites may cause the experimental animals cancerization, genic mutation, reproductivetoxicity and also be harmful to humans. Therefore, the detection of nitrofurans is critical in food security. Herein a method was developed for the determination of four kinds of nitrofurans using liquid chromatography-tandem mass spectrometry （LC-MS）. After derivatization with 2-nitrobenzene formaldehyde in acidic conditions, nitrofurans metabolites can be extracted and purified by ethyl acetate, and detected by liquid chromatography-tandem mass spectrometry after electrospray ionization. The results indicated that the chromatographic peak area has a good linear relationship with concentration of the four nitrofurans in the range of 0.1-10.0 ng / mL. The method is simple, sensitive and accurate.
