CAJ | 학술논문

FecB是19世纪80年代在布鲁拉美利奴（Booroola Merino）绵羊中发现的能增加排卵数和产羔数的一个常染色体突变基因,是在绵羊中识别出的第一个高繁殖力主效基因。本研究利用PCR-SSCP以及PCR-RFLP方法对湖羊、小尾寒羊、阿勒泰羊、洼地绵羊共470只的FecB基因进行单核苷酸多态性分析,验证FecB基因为绵羊的高繁殖力的主效基因,并以洼地绵羊为研究对象,检验FecB基因是否为洼地绵羊高繁殖力的主效基因。实验结果表明上述两种方法均能准确判型,稳定可靠性良好,并且判断的结果一致。研究结果显示：上述4个绵羊品种均携带FecB突变,且能证明FecB基因为洼地绵羊高繁殖力的主效基因。
FecB시19세기80년대재포로랍미리노（Booroola Merino）면양중발현적능증가배란수화산고수적일개상염색체돌변기인,시재면양중식별출적제일개고번식력주효기인。본연구이용PCR-SSCP이급PCR-RFLP방법대호양、소미한양、아륵태양、와지면양공470지적FecB기인진행단핵감산다태성분석,험증FecB기인위면양적고번식력적주효기인,병이와지면양위연구대상,검험FecB기인시부위와지면양고번식력적주효기인。실험결과표명상술량충방법균능준학판형,은정가고성량호,병차판단적결과일치。연구결과현시：상술4개면양품충균휴대FecB돌변,차능증명FecB기인위와지면양고번식력적주효기인。
The Booroola gene（FecB） is a dominant autosomal gene responsible for the fecundity of Booroola Merino sheep with an additive effect on ovulation rate and litter size firstly identified in 1980s.In this study,PCR-SSCP,PCR-RFLP and DNA sequences were used to detect the polymorphism of mononucleotide within FecB gene of 470 sheep including Hu sheep,Small Tailed Han sheep,Wadi sheep,and Altay sheep.And detected whether the FecB gene was the major gene for prolificacy to wadi sheep.The results indicated that these two methods had the identical results and displayed accurately genotyping,good stability and reliability.And the result also showed that the four sheep breeds or strains carried the same FecB mutation（A746G） as found in Booroola Merino sheep.Which is more important,it also proved that FecB gene could be the major gene for prolificacy to wadi sheep.