陕西师范大学学报:自然科学版
陝西師範大學學報:自然科學版
협서사범대학학보:자연과학판
Journal of Shaanxi Normal University: Nat Sci Ed
2012年
2期
67-70,75
,共5页
张小静%郑哲民%赵玲%孙慧敏%许姝娟%李鑫
張小靜%鄭哲民%趙玲%孫慧敏%許姝娟%李鑫
장소정%정철민%조령%손혜민%허주연%리흠
蝗总科%28SrDNA%分子系统树%系统发育
蝗總科%28SrDNA%分子繫統樹%繫統髮育
황총과%28SrDNA%분자계통수%계통발육
acridoidea%28SrDNA%phylogenetic tree%phylogeny
基于分子系统学方面对蝗总科7科20种昆虫的系统发育进行了探讨.采用PCR产物直接测序法测定了该20种昆虫的核基因28SrDNA的部分片段序列,并从GenBank中下载的摩门螽蟖和变色乌蜢作为外群,采用最大简约法(MP)构建了分子系统树.在获得的504bp序列中,有340个保守位点,161个变异位点,45个简约信息位点;G+C约占69.8%,A+T约占30.2%.分子系统树表明:蝗总科7科中槌角蝗科、网翅蝗科和剑角蝗科的分类地位有待进一步的研究,而其他科的分类地位基本与形态学分类一致.
基于分子繫統學方麵對蝗總科7科20種昆蟲的繫統髮育進行瞭探討.採用PCR產物直接測序法測定瞭該20種昆蟲的覈基因28SrDNA的部分片段序列,併從GenBank中下載的摩門螽蟖和變色烏蜢作為外群,採用最大簡約法(MP)構建瞭分子繫統樹.在穫得的504bp序列中,有340箇保守位點,161箇變異位點,45箇簡約信息位點;G+C約佔69.8%,A+T約佔30.2%.分子繫統樹錶明:蝗總科7科中槌角蝗科、網翅蝗科和劍角蝗科的分類地位有待進一步的研究,而其他科的分類地位基本與形態學分類一緻.
기우분자계통학방면대황총과7과20충곤충적계통발육진행료탐토.채용PCR산물직접측서법측정료해20충곤충적핵기인28SrDNA적부분편단서렬,병종GenBank중하재적마문종사화변색오맹작위외군,채용최대간약법(MP)구건료분자계통수.재획득적504bp서렬중,유340개보수위점,161개변이위점,45개간약신식위점;G+C약점69.8%,A+T약점30.2%.분자계통수표명:황총과7과중퇴각황과、망시황과화검각황과적분류지위유대진일보적연구,이기타과적분류지위기본여형태학분류일치.
Partial species of Acridoidea (20 species, 7 families)were studied in molecularsystematics. The partial 28SrDNA gene sequence Anabrus simplex and Erianthus versicolor were relationships of these species from family level were (MP) method. There were 340 conserved sites, 161 sites, and G+ C was about 69.8% and A + T was only about 30.2 % in the 504 nucleotides of the data. The results showed that taxonomic status of some specie, of Gomphoceridae, Arcypteridae and Acrididae should be researched further, however, taxonomic status of some species of other 4 families are consistent with morphological taxonomy in Acridoidea.