CAJ | 학술논문

用鲤鱼表皮瘤细胞系（epithelioma papulosum cyprini cell line，EPC）从福建省某美洲牛蛙养殖场分离到一株病毒FJ049。感染病毒的EPC呈现细胞圆缩、颗粒增多、脱落等特征性病变。间接免疫荧光检测结果表明，感染FJ049的EPC细胞与虹彩病毒单克隆抗体反应并出现特异性的胞浆荧光。采用PCR对虹彩病毒主衣壳蛋白（major capsid protein，MCP）基因保守区域进行扩增，扩增出531bp的特异性基因片段。MCP基因同源性和遗传进化分析结果表明分离株FJ049与沼泽绿牛蛙虹彩病毒RGV9808的核苷酸同源性最高，为99．8％，属于虹彩病毒科蛙病毒属。
용리어표피류세포계（epithelioma papulosum cyprini cell line，EPC）종복건성모미주우와양식장분리도일주병독FJ049。감염병독적EPC정현세포원축、과립증다、탈락등특정성병변。간접면역형광검측결과표명，감염FJ049적EPC세포여홍채병독단극륭항체반응병출현특이성적포장형광。채용PCR대홍채병독주의각단백（major capsid protein，MCP）기인보수구역진행확증，확증출531bp적특이성기인편단。MCP기인동원성화유전진화분석결과표명분리주FJ049여소택록우와홍채병독RGV9808적핵감산동원성최고，위99．8％，속우홍채병독과와병독속。
An iridovims strain F J049 was isolated on epithelioma papillosum cyprinid （EPC） cell line from a moribund American bullfrog （Rana catesbiana） on a farm in Fujian Province. The infected EPC cells developed cytopathogenic effects （CPE）, such as rounding, granulation and detachment. The monoclonal antibody against iridovirus showed positive reaction in the FJ049-infected EPC cells in indirect immunofluorescence assay and specific fluorescence appeared in the cytoplasm of infected cells. A specific 53 lbp fragment was amplified in PCR from the conserved regions of major capsid protein （MCP） gene. The phylogenetic analysis ofMCP gene showed that the FJ049 strain shared 99.8% identity with the RGV9808 strain isolated from Rana grylio stejneger, classified in genus Ranavirus, family Iridoviridae.