CAJ | 학술논문

为研究水孔蛋白基因EgPIP1；3在洋桔梗（Eustoma grandifrum）采后切花中的表达特点，采用肌动蛋白（β-actin）基因为内参，以洋桔梗花瓣cDNA为模板，通过对PCR体系循环数及退火温度的优化，建立了一个稳定、特异的半定量反转录PCR（Reverse transcriptionPCR，RT-PCR）体系．运用此体系检测了EgPIP1；3在洋桔梗切花中的表达．结果表明，EgPIP1；3基因在洋桔梗切花的茎、叶、萼、花瓣、雄蕊和雌蕊中均有表达，但表达量存在明显的差异，依次表现为茎〉雌蕊〉萼〉叶〉雄蕊〉花瓣．
위연구수공단백기인EgPIP1；3재양길경（Eustoma grandifrum）채후절화중적표체특점，채용기동단백（β-actin）기인위내삼，이양길경화판cDNA위모판，통과대PCR체계순배수급퇴화온도적우화，건립료일개은정、특이적반정량반전록PCR（Reverse transcriptionPCR，RT-PCR）체계．운용차체계검측료EgPIP1；3재양길경절화중적표체．결과표명，EgPIP1；3기인재양길경절화적경、협、악、화판、웅예화자예중균유표체，단표체량존재명현적차이，의차표현위경〉자예〉악〉협〉웅예〉화판．
A stable and specific semi-quantitative reverse transcription PCR （RT-PCR） system for the expression analysis of EgPIP1;3 gene in different organs of cut eustoma （Eustoma grandiforum） flowers was established, using β-actin gene as the internal control, and the eDNA from eustoma petal as the template. Cycle numbers and annealing temperature for this system were optimized. Based on the sys- tem, the expression of EgPIP1; 3 gene in different tissues of cut eustoma flowers was analyzed. The re- suits showed that the gene could be expressed in all detected tissues of cut eustoma flowers, including stem, leaf, calyx, petal, stamen and pistil. However, there were significant differences in mRNA lev- els among them. The highest expression level was detected in stems, then immediately followed by pis- tils, while moderate expression levels were in calyxes, leaves and stamina, and the lowest level was in petals.