CAJ | 학술논문

采用酸醇提取减压浓缩法提取猪胰脏中的胰岛素。以胰岛素的提取含量为考察指标，以提取所用的溶剂、除杂质时的碱化、酸化pH值为考察因素进行正交实验，得出了最佳提取工艺条件为：以丙酮、水、氯化钙为提取溶剂，酸化pH为3．8，碱化pit为8．0除杂。优选得到的工艺稳定可行且提取率最高而杂质含量少。
채용산순제취감압농축법제취저이장중적이도소。이이도소적제취함량위고찰지표，이제취소용적용제、제잡질시적감화、산화pH치위고찰인소진행정교실험，득출료최가제취공예조건위：이병동、수、록화개위제취용제，산화pH위3．8，감화pit위8．0제잡。우선득도적공예은정가행차제취솔최고이잡질함량소。
Using acid alcohol extraction and reduced pressure concentration method to extract insulin from pig pancreas, and carrying out the orthogonal experiment with insulin extraction content as examination target, extracting solvent and pH value of impurity removal as examination facts. And the optimum extracting conditions are as following： acetone, water and calcium chloride as extracting solvent, acidified pH value is 3.8 and alkalify pH value is 8.0. The optimum extracting process with highest extraction rate and least impurity content is stable and feasible.