贵阳学院学报:自然科学版
貴暘學院學報:自然科學版
귀양학원학보:자연과학판
Journal of Guiyang College:Natural Science
2012年
3期
44-46
,共3页
杨碧仙%袁德刚%吴吉超%滚兴圣
楊碧仙%袁德剛%吳吉超%滾興聖
양벽선%원덕강%오길초%곤흥골
香樟根%总黄酮%提取%正交试验
香樟根%總黃酮%提取%正交試驗
향장근%총황동%제취%정교시험
Cinnamomum camphora rootes%Total flavonoids%Extraction%Orthogonal test
[目的]采用乙醇提取法,优选香樟根总黄酮的提取工艺。[方法]选择乙醇浓度、料液比、提取温度、提取时间为考察因素,采用正交设计L9(34)进行提取工艺优选试验。[结果]回归方程为:Y=13.68X-0.0027,r2=0.998,芦丁浓度在0.0—0.04516mg/mL范围内有良好线性关系,最佳提取工艺为:乙醇的体积分数为50%,料液比为1:20,温度为70℃,提取时间为2h。[结论]优选的提取工艺简单、易行,可作为开发香樟根制剂的依据。
[目的]採用乙醇提取法,優選香樟根總黃酮的提取工藝。[方法]選擇乙醇濃度、料液比、提取溫度、提取時間為攷察因素,採用正交設計L9(34)進行提取工藝優選試驗。[結果]迴歸方程為:Y=13.68X-0.0027,r2=0.998,蘆丁濃度在0.0—0.04516mg/mL範圍內有良好線性關繫,最佳提取工藝為:乙醇的體積分數為50%,料液比為1:20,溫度為70℃,提取時間為2h。[結論]優選的提取工藝簡單、易行,可作為開髮香樟根製劑的依據。
[목적]채용을순제취법,우선향장근총황동적제취공예。[방법]선택을순농도、료액비、제취온도、제취시간위고찰인소,채용정교설계L9(34)진행제취공예우선시험。[결과]회귀방정위:Y=13.68X-0.0027,r2=0.998,호정농도재0.0—0.04516mg/mL범위내유량호선성관계,최가제취공예위:을순적체적분수위50%,료액비위1:20,온도위70℃,제취시간위2h。[결론]우선적제취공예간단、역행,가작위개발향장근제제적의거。
Objective: Using of ethanol extraction to Optimize extraction technology of total Flavonoids from Cinnamomum camphora roots. Method:Through ethanol concentration , the solid liquid ratio, extraction temperature and extraction time as the study factors, the optimum process for extracting the flavonoids by Orthogonal design L9 (3^4) . Resuhs:The regression equation was Y = 13. 68X -0. 0027 ,r2 =0.99 8 ,confirming a good linear relationship between the concentration and absorbance of rutin in the range of 0. 0 - 0. 04516mg · mL-1 , the optimum conditions of extracting were 50% ethanol, solid liquid ratio is 1:20, extraction temperature is 70℃: , extraction time is 2 h. Conclusion:Optimization of the extraction process is simple and easy , could be used the basis for the development of camphor root preparations.