贵阳学院学报:自然科学版
貴暘學院學報:自然科學版
귀양학원학보:자연과학판
Journal of Guiyang College:Natural Science
2012年
3期
41-43
,共3页
麻鸭血%HBsAg检测%ELISA法%校内实训%应用
痳鴨血%HBsAg檢測%ELISA法%校內實訓%應用
마압혈%HBsAg검측%ELISA법%교내실훈%응용
Ma duck blood%HBsAg test%ELISA the training%courses application
为了避免实验室生物安全事故(学生感染和实验室污染)的发生,探讨采用麻鸭血替代人血用酶联免疫吸附试验(ELISA法)作乙肝病毒表面抗原(HBsAg)检测的可行性。方法:收集黔东南地区100份麻鸭血标本,采用ELISA法检测HBsAg。结果 100份麻鸭血清标本HBsAg检测结果均为阴性。结论:我国多地报道麻鸭血携带鸭乙肝病毒(DHBV),但通过检测,我地区麻鸭血HBsAg为阴性,在校内实训课中可作为标本应用。
為瞭避免實驗室生物安全事故(學生感染和實驗室汙染)的髮生,探討採用痳鴨血替代人血用酶聯免疫吸附試驗(ELISA法)作乙肝病毒錶麵抗原(HBsAg)檢測的可行性。方法:收集黔東南地區100份痳鴨血標本,採用ELISA法檢測HBsAg。結果 100份痳鴨血清標本HBsAg檢測結果均為陰性。結論:我國多地報道痳鴨血攜帶鴨乙肝病毒(DHBV),但通過檢測,我地區痳鴨血HBsAg為陰性,在校內實訓課中可作為標本應用。
위료피면실험실생물안전사고(학생감염화실험실오염)적발생,탐토채용마압혈체대인혈용매련면역흡부시험(ELISA법)작을간병독표면항원(HBsAg)검측적가행성。방법:수집검동남지구100빈마압혈표본,채용ELISA법검측HBsAg。결과 100빈마압혈청표본HBsAg검측결과균위음성。결론:아국다지보도마압혈휴대압을간병독(DHBV),단통과검측,아지구마압혈HBsAg위음성,재교내실훈과중가작위표본응용。
purpose In order to avoid the accidents (infection on the students and pollution to the lab) in the biological lab, we have explored the possibility of testing Hepatitis B virsu Surface Anti C, en(HbsAg) in the way of Enzyme Linked Immunosorbent Assay ( ELISA), with the Ma Duck blood replacing the human blood. Methodology Collect the blood from 100 Ma ducks in the area of Qiandongnan as the samples, then test HBsAg using the way of ELISA. Results All the blood samples from the 100 Ma ducks show the test resuls are negative. Conclusion As reported, Ma duck blood carries the Duck Hepatitis B Virus (DHBV) in most areas of China, however, Ya duck blood in the area of Qiandongnan is negative in the test, so it can be appiled for samples in the training courses.