CAJ | 학술논문

目的探讨传染性胰脏坏死病毒（IPNV）在虹鳟鱼中的存在与传播，为我国北方地区IPNV疫情动态监测和防控提供依据。方法从辽宁省某渔场中患传染性胰脏坏死病的虹鳟鱼中分离出1株优势毒株，对新分离出来的菌株进行培养增殖、感染试验及理化性质的鉴定。结果新分离的IPNV在CHSE细胞上增殖良好，且可引起良好的病变，病毒滴度达10^-805／0．1mL。分离毒对酸、碱和热均稳定，对乙醚不敏感，病毒的复制不被FUDR抑制。根据IPNV的保守基因N基因序列，设计特异性引物，结果扩增出224bp的片段。对该片段进行测序分析，发现与IPNV的参考序列有较高的相似性，表明该毒株为传染性胰脏坏死病毒。结论IPNV已在北方虹鳟鱼中存在，可为我国北方IPNV疫情动态监测和防控提供依据。
목적탐토전염성이장배사병독（IPNV）재홍준어중적존재여전파，위아국북방지구IPNV역정동태감측화방공제공의거。방법종요녕성모어장중환전염성이장배사병적홍준어중분리출1주우세독주，대신분리출래적균주진행배양증식、감염시험급이화성질적감정。결과신분리적IPNV재CHSE세포상증식량호，차가인기량호적병변，병독적도체10^-805／0．1mL。분리독대산、감화열균은정，대을미불민감，병독적복제불피FUDR억제。근거IPNV적보수기인N기인서렬，설계특이성인물，결과확증출224bp적편단。대해편단진행측서분석，발현여IPNV적삼고서렬유교고적상사성，표명해독주위전염성이장배사병독。결론IPNV이재북방홍준어중존재，가위아국북방IPNV역정동태감측화방공제공의거。
Objective： To explore the presence and spreading of infectious pancreatic necrosis virus （IPNV） in rainbow trout so as to provide basis for dynanamic surveillance and control of IPN in the onorth pants of chine.Methods： A dominant virus strain was isolated from IPN infected rainbow trouts in a fish farm in Liaoning province.The strain was a subjected to culture, qrowth, infective test and physical and chemical identification.Results： The newly isolated IPNV grew well in CHSE cell, inducing very good PCE with a titer of 1085/0.1 mL.The virus was stable to acid, base and heat, unsensitive to ether and its replication was not prohibitted with FUDR.224 bp fragments were obtained through amplification with the specific primers designed according to the conservative N gene sequence of IPNV. The fragment was found to be of high similarity to the reference sequearce of IPNV.Conclusion： IPNV has present in rainbow trout in the north parts of china, thas providag basis for IPNV surveillance and control.