CAJ | 학술논문

在常规酶联免疫法（ELISA）的基础上，用辣根过氧化物酶标记氯霉素与牛血清白蛋白偶联后免疫小鼠制得的单克隆抗体，并优化抗体工作浓度、反应时间等试验参数，建立了一种简便、快速、准确的氯霉素（CAP）残留一步式化学发光酶联免疫检测方法。结果表明：该方法最佳检测范围为20～1620pg／mL，IC50值达59．911pg／mL，检测限为9．3Pg／g，可检出国际规定的CAP残留标准限量值以下的残留量。与常规ELISA检测方法相比，不仅提高了检测限和灵敏度，而且反应时间缩短了60min。采用一步式化学发光酶联免疫检测氯霉素残留，可以大幅度缩减检测时间，更符合快速检测的要求。
재상규매련면역법（ELISA）적기출상，용랄근과양화물매표기록매소여우혈청백단백우련후면역소서제득적단극륭항체，병우화항체공작농도、반응시간등시험삼수，건립료일충간편、쾌속、준학적록매소（CAP）잔류일보식화학발광매련면역검측방법。결과표명：해방법최가검측범위위20～1620pg／mL，IC50치체59．911pg／mL，검측한위9．3Pg／g，가검출국제규정적CAP잔류표준한량치이하적잔류량。여상규ELISA검측방법상비，불부제고료검측한화령민도，이차반응시간축단료60min。채용일보식화학발광매련면역검측록매소잔류，가이대폭도축감검측시간，경부합쾌속검측적요구。
A convenient, rapid and accurate one step chloramphenicol （CAP） residue chemiluminescent enzyme immunoassay（CLEIA） detection method was established based on conventional ELISA, by using HRP labeled monoclonal antibodies obtained from immunizing mice that were injected with CAP - BSA coupling protein, and optimizing test parameters, such as antibody concentration, reaction time. The results showed that the best detection range of this method was 20 -1620 pg/mL, the ICs0 was 59. 911 pg/mL, the detection limit was 9.3 pg/g. It can check out the international provisions standard limited value of the CAP residue. Compared with conventional ELISA test method, the CLEIA method can not only increase the detection limit and sensitivity, but also shorten the reaction time by 60 minutes. It indicated that using the one step chemiluminescent enzyme immunoassay kit to detect chloramphenicol residue can decrease time consuming, and was more appropriate for fast detection requirements.