贵州师范大学学报：自然科学版
貴州師範大學學報：自然科學版
귀주사범대학학보：자연과학판
Journal of Guizhou Normal University(Natural Sciences)
2012年
2期
86-89
,共4页
周燚%方彩华%李良鸣%石家瑾
週燚%方綵華%李良鳴%石傢瑾
주일%방채화%리량명%석가근
间歇低氧%糖尿病大鼠%琥珀酸脱氢酶%乳酸脱氢酶
間歇低氧%糖尿病大鼠%琥珀痠脫氫酶%乳痠脫氫酶
간헐저양%당뇨병대서%호박산탈경매%유산탈경매
intermittent hypoxia%diabetic rats%SDH%LDH
目的：探讨间歇低氧干预对糖尿病大鼠骨骼肌糖代谢酶活性的影响。方法：采用长期高糖高脂膳食加小剂量链脲佐菌素（STZ）的方法建立糖尿病大鼠模型，将复制成功的模型大鼠随机分为模型对照组与低氧干预组。模型对照组在常氧环境下正常生活，低氧干预组大鼠每天在低氧帐篷内低氧暴露1h（O2浓度15．4±0．2％）、每周休息1d、共干预28d后36h取股四头肌测试琥珀酸脱氢酶（SDH）、乳酸脱氢酶（LDH）活性。结果：模型对照组大鼠股四头肌SDH活性显著低于正常对照组（P〈0．05），LDH活性高于正常对照组（P〉0．05），低氧干预组SDH、LDH活性与模型对照组比较均无明显差异（P〉0．05）。结论：实验性糖尿病大鼠骨骼肌SDH活性降低，有氧氧化供能能力下降，而LDH活性增强，无氧酵解能力提高；本实验所设计的间歇低氧干预模式对实验性糖尿病大鼠骨骼肌SDH、LDH活性均无明显积极作用。
目的：探討間歇低氧榦預對糖尿病大鼠骨骼肌糖代謝酶活性的影響。方法：採用長期高糖高脂膳食加小劑量鏈脲佐菌素（STZ）的方法建立糖尿病大鼠模型，將複製成功的模型大鼠隨機分為模型對照組與低氧榦預組。模型對照組在常氧環境下正常生活，低氧榦預組大鼠每天在低氧帳篷內低氧暴露1h（O2濃度15．4±0．2％）、每週休息1d、共榦預28d後36h取股四頭肌測試琥珀痠脫氫酶（SDH）、乳痠脫氫酶（LDH）活性。結果：模型對照組大鼠股四頭肌SDH活性顯著低于正常對照組（P〈0．05），LDH活性高于正常對照組（P〉0．05），低氧榦預組SDH、LDH活性與模型對照組比較均無明顯差異（P〉0．05）。結論：實驗性糖尿病大鼠骨骼肌SDH活性降低，有氧氧化供能能力下降，而LDH活性增彊，無氧酵解能力提高；本實驗所設計的間歇低氧榦預模式對實驗性糖尿病大鼠骨骼肌SDH、LDH活性均無明顯積極作用。
목적：탐토간헐저양간예대당뇨병대서골격기당대사매활성적영향。방법：채용장기고당고지선식가소제량련뇨좌균소（STZ）적방법건립당뇨병대서모형，장복제성공적모형대서수궤분위모형대조조여저양간예조。모형대조조재상양배경하정상생활，저양간예조대서매천재저양장봉내저양폭로1h（O2농도15．4±0．2％）、매주휴식1d、공간예28d후36h취고사두기측시호박산탈경매（SDH）、유산탈경매（LDH）활성。결과：모형대조조대서고사두기SDH활성현저저우정상대조조（P〈0．05），LDH활성고우정상대조조（P〉0．05），저양간예조SDH、LDH활성여모형대조조비교균무명현차이（P〉0．05）。결론：실험성당뇨병대서골격기SDH활성강저，유양양화공능능력하강，이LDH활성증강，무양효해능력제고；본실험소설계적간헐저양간예모식대실험성당뇨병대서골격기SDH、LDH활성균무명현적겁작용。
Objective The purpose of this study was to investigate the effect of intermittent hypoxia on the SDH and LDH activities in skeletal muscle of diabetic rats. Methods The diabetic rat model was established by long-term high sugar and fat diet with low dose （30mg/kg body weight） of the streptozocin （STZ） by intraperitoneal injection only once. The diabetic rats were randomly divided into model control （MC） group and hypoxia interfering （HI） group. The MC rats were living in normal environment while the HI rats were exposed to hypoxia for one hour per day in hypoxic tent （oxygen concentration 15.4 ±0. 2 % ）, six days a week for four weeks. The activities of SDH and LDH in the quadriceps femoris rats were measured. Results Compared with the rats in normal control group, the SDH activity reduced significantly （ P 〈 0. 05 ） and LDH activity increased only slightly in model control group. No significant differences of the SDH and LDH activities were found between HI and MC group. Conclu- sion The study suggested that the aerobic oxidative capability reduced and glycolytic capability increased in skeletal muscle of diabetic rats. Hypoxia interfering for four weeks did not have effect on the SDH and LDH activities in skeletal muscle of diabetic rats.
