CAJ | 학술논문

[目的]克隆南瓜基因CmNAC并进行序列分析。[方法]以南瓜叶片为材料,根据甘蓝型油菜NAC1、番茄NAC和辣椒NAC保守结构域设计一对简并引物,采用RT-PCR方法扩增得出长度约为440bp大小的DNA片段,将其克隆至pMDl9-T载体上,对重组克隆进行测序,用BLAST和DNAMAN软件对核酸及氨基酸序列进行分析。[结果]所获得的南瓜NAC基因片段由442个碱基组成,编码147个氨基酸,命名为CmNAC;该基因片段具有其它植物NAC基因中存在的保守区,并且属于NAC家族中ATAF1/2亚家族。[结论]实验拟在南瓜中获得和抗逆性相关的NAC基因,为进一步研究该基因的生物学功能和植物基因工程奠定理论基础。
[목적]극륭남과기인CmNAC병진행서렬분석。[방법]이남과협편위재료,근거감람형유채NAC1、번가NAC화랄초NAC보수결구역설계일대간병인물,채용RT-PCR방법확증득출장도약위440bp대소적DNA편단,장기극륭지pMDl9-T재체상,대중조극륭진행측서,용BLAST화DNAMAN연건대핵산급안기산서렬진행분석。[결과]소획득적남과NAC기인편단유442개감기조성,편마147개안기산,명명위CmNAC;해기인편단구유기타식물NAC기인중존재적보수구,병차속우NAC가족중ATAF1/2아가족。[결론]실험의재남과중획득화항역성상관적NAC기인,위진일보연구해기인적생물학공능화식물기인공정전정이론기출。
[Objective] The aim was to clone the CmNAC gene from Cucurbita moschata and analyze the sequence characteristics. [Method] A pair of degenerate primers was designed based on the conserved sequences of NAC gene from Brassica napus, Lycopersicon esculentum and Capsicum annuum. NAC transcription factor gene was amplified by RT-PCR from Cucurbita moschata leaves and cloned into pMD-19T vector; then the recombinant clones were sequenced. Finally, the sequences of nucleic acid and amino acid were analyzed using BLAST and DNAMAN software. [Result] The NAC transcription factor gene cloned from C. moschata included 442 bp encoding 147 amino acids, named CmNAC. The NAC gene fragment contained a conserved region like other plant NAC genes and belonged to the NAC family ATAF1/2 subfamily. [Conclusion] The stress resistance related gene NAC cloned from C. moschata is a foundation for further study on the biological function of the gene and plant genetic engineering.