CAJ | 학술논문

[目的]利用小分子DNA鉴定重金属离子对DNA的损伤。[方法]将pUC18DNA分子暴露于Hg2＋、Cr6＋、Pb2＋、Cd2＋4种重金属离子环境中,经一定时间处理后对DNA分子进行生物活性研究,并用凝胶电泳和增色效应分析了重金属离子对DNA分子生物活性影响机制。[结果]DNA活性分析表明,4种重金属对pUC18的影响程度为Hg2＋〉Cr6＋〉Pb2＋〉Cd2＋;凝胶电泳和增色效应结果表明,4种重金属离子主要是引起DNA分子交联导致其生物活性的降低,交联程度为Hg2＋〉Cr6＋〉Pb2＋〉Cd2＋。[结论]该研究表明,pUC18DNA分子可用作鉴定重金属离子对DNA损伤的依据,为评价重金属对DNA毒害作用提供了一种简捷有效的技术方法。
[목적]이용소분자DNA감정중금속리자대DNA적손상。[방법]장pUC18DNA분자폭로우Hg2＋、Cr6＋、Pb2＋、Cd2＋4충중금속리자배경중,경일정시간처리후대DNA분자진행생물활성연구,병용응효전영화증색효응분석료중금속리자대DNA분자생물활성영향궤제。[결과]DNA활성분석표명,4충중금속대pUC18적영향정도위Hg2＋〉Cr6＋〉Pb2＋〉Cd2＋;응효전영화증색효응결과표명,4충중금속리자주요시인기DNA분자교련도치기생물활성적강저,교련정도위Hg2＋〉Cr6＋〉Pb2＋〉Cd2＋。[결론]해연구표명,pUC18DNA분자가용작감정중금속리자대DNA손상적의거,위평개중금속대DNA독해작용제공료일충간첩유효적기술방법。
[Objective] The aim was to establish a convenient and effective method to evaluate the toxicity of heavy metal ions by using small molecular DNA. [Method] pUC18 DNA which had exposed to the four heavy metal ions of Hg2＋, Cr6＋, Pb2＋, Cd2＋ was used to study the bioactivity of DNA; simultaneously, gel electrophoresis and hyperchromic effect were employed to detect the mechanism of DNA damage. [Result] The bioactivity of the exposed DNA was decreased and the influence degree was Hg2＋Cr6＋Pb2＋Cd2＋; the gel electrophoresis and hyperchromic effect proved that the main reason leading to reduce the bioactivity was DNA cross link, in the order pf Hg2＋Cr6＋Pb2＋Cd2＋. [Conclusion] The study indicated that pUC18 DNA could be used to assay the damage of DNA causing by heavy mental ions, which may be a potential, simple and effective tool to evaluate toxicity of heavy metal ions to DNA.