CAJ | 학술논문

[目的]探讨紫鸭趾草在铜胁迫下部分基因的生物学功能,从分子生物学的角度研究植物的耐铜机理,拟通过该研究解决铜污染问题。[方法]以具有铜离子富集能力的植物紫鸭跖草（Setcreasea purpurea Boom）为试验材料。通过cDNA-AFLP以及银染技术,获得90个差异表达片段,扩增得到其中17个片段,经纯化鉴定最终获得6个差异片段的序列,并对其进行了BLASTX比对。[结果]6条差异表达片段可能在铜离子对紫鸭趾草的胁迫中产生作用。其中编号为E5MG-3的差异序列与拟南芥mRNA序列（登录号为AAM62956.1）同源性达49%;E4MB-2与马铃薯mRNA序列（登录号为A5A7I7.1）同源性达53%;E6MG-1与芋mRNA序列（登录号AAO62313.1）同源性达65%。由此推测差异表达基因与细胞信号传导、抗氧化、新陈代谢以及蛋白质修饰等有关。[结论]该研究为进一步揭示紫鸭趾草铜胁迫响应的调控网络奠定基础。
[목적]탐토자압지초재동협박하부분기인적생물학공능,종분자생물학적각도연구식물적내동궤리,의통과해연구해결동오염문제。[방법]이구유동리자부집능력적식물자압척초（Setcreasea purpurea Boom）위시험재료。통과cDNA-AFLP이급은염기술,획득90개차이표체편단,확증득도기중17개편단,경순화감정최종획득6개차이편단적서렬,병대기진행료BLASTX비대。[결과]6조차이표체편단가능재동리자대자압지초적협박중산생작용。기중편호위E5MG-3적차이서렬여의남개mRNA서렬（등록호위AAM62956.1）동원성체49%;E4MB-2여마령서mRNA서렬（등록호위A5A7I7.1）동원성체53%;E6MG-1여우mRNA서렬（등록호AAO62313.1）동원성체65%。유차추측차이표체기인여세포신호전도、항양화、신진대사이급단백질수식등유관。[결론]해연구위진일보게시자압지초동협박향응적조공망락전정기출。
[Objective] The aim was to explore biological function of related genes in Setcreasea purpurea Boom under copper stress and to study the mechanism of its copper-resistance from standpoint of molecular biology in order to solve the problem of copper pollution. [Method] Setcreasea purpurea Boom was taken as experimental material which enjoys enrichment ability to cupric ion. About 90 fragments of differential expression were obtained by cDNA-AFLP and silver staining technique, among which, 17 fragments were amplified. After purification and identification, sequences of 6 differential fragments were got and used for BLAST X contrast. [Result] Six differential expressed fragments may play roles when Setcreasea purpurea Boom was under copper stress. The homology achieved 49% between differential sequences of E5MG-3 and of Arabidopsis thaliana mRNA （accession numbers： AAM62956.1）, homology was 53% between sequences of E4MB-2 and Solanum tuberosum mRNA （accession numbers： A5A717.1）, and 65% between sequences of E6MG-1 and Colocasia esculenta （L.） Schott mRNA （accession numbers： AAO62313.1）. It can be concluded that differential expressed genes are related to cell signaling, antioxidation, metabolism and protein modification. [Conclusion] The study has laid foundation for further exploration of regulatory network about response of Setcreasea purpurea Boom to copper stress.