CAJ | 학술논문

[目的]研究将唯一栖息于红树林的两栖动物--海蛙（Rana cancrivora）作为红树林环境质量监测指示物种的可行性。[方法]于2009年7月和8月在海南东寨港红树林国家级自然保护区的码头和红树林核心区（简称红树林）2个不同微生境中采集海蛙,检测分析其肝脏和肌肉中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、乙酰胆碱脂酶（AChE）和诱导型一氧化氮合成酶（iNOS）活性及丙二醛（MDA）含量,同时测量计算其肝体指数、肾体指数和脾体指数,以综合评估海蛙所受环境胁迫大小,并用以评估红树林不同生境质量好坏。[结果]红树林生境中海蛙的肝脏、肾脏和脾脏系数均低于码头,但仅肾脏系数差异显著（P〈0.05）;该生境中海蛙组织的4种酶活性均高于码头,且SOD和CAT活性均表现出差异显著（P〈0.05）;同时MDA含量也显著低于码头（P〈0.05）。结果表明红树林生境中海蛙的抗氧化酶活性高于码头个体,相应地脂质过氧化程度低,所受环境胁迫程度低于码头。[结论]由于人类干扰和旅游开发等因素使码头生境质量明显低于红树林核心区,而红树林生境中海蛙个体所受环境胁迫小于码头个体,与这2个微生境中的生境质量相一致,表明海蛙可作为红树林环境质量评价的指示物种。
[목적]연구장유일서식우홍수림적량서동물--해와（Rana cancrivora）작위홍수림배경질량감측지시물충적가행성。[방법]우2009년7월화8월재해남동채항홍수림국가급자연보호구적마두화홍수림핵심구（간칭홍수림）2개불동미생경중채집해와,검측분석기간장화기육중초양화물기화매（SOD）、과양화경매（CAT）、을선담감지매（AChE）화유도형일양화담합성매（iNOS）활성급병이철（MDA）함량,동시측량계산기간체지수、신체지수화비체지수,이종합평고해와소수배경협박대소,병용이평고홍수림불동생경질량호배。[결과]홍수림생경중해와적간장、신장화비장계수균저우마두,단부신장계수차이현저（P〈0.05）;해생경중해와조직적4충매활성균고우마두,차SOD화CAT활성균표현출차이현저（P〈0.05）;동시MDA함량야현저저우마두（P〈0.05）。결과표명홍수림생경중해와적항양화매활성고우마두개체,상응지지질과양화정도저,소수배경협박정도저우마두。[결론]유우인류간우화여유개발등인소사마두생경질량명현저우홍수림핵심구,이홍수림생경중해와개체소수배경협박소우마두개체,여저2개미생경중적생경질량상일치,표명해와가작위홍수림배경질량평개적지시물충。
[Objective] The aim was to study on the feasibility to take Rana cancrivora, the only amphibian inhabiting mangrove, as indicator species for environment monitoring of mangrove. [Methods] Rana cancrivorae were collected in July and August of 2009 from two different microhabitats, including the pier and the core mangrove area of National Dongzhai Harbor Mangrove Reserve in Hainan Province. In addition, examination and analysis were conducted on activity of superoxide dismutase （SOD）, catalase （CAT）, acetylcholine esterase （AChE）, and inducible nitric oxide synthase （iNOS）, and content of malonaldehyde （MDA） in its liver and muscle. Furthermore, indices of hepar/body, kidney/body and spleen/body were measured to make a comprehensive evaluation on Rana cancrivora stress from environment and mangrove quality in different microhabitats. [Result] In mangrove habitat, indices of hepar/body, kidney/body and spleen/body of Rana cancrivora were all lower than that in pier and only index of kidney/body differed significantly （P0.05）; the four enzyme activities were all higher than that in pier and activities of SOD and CAT differed significantly （P0.05）. In addition, MDA content was lower than that in pier significantly （P0.05）. The result indicated that antioxidant enzyme activity of Rana cancrivora in mangrove habitat was higher than that in pier individually, lipid peroxidation and the stress were lower correspondingly. [Conclusion] Because of human intervention and travelling development, quality of pier habitat was lower than that in mangrove core area, and stress for Rana cancrivora by environment was smaller than that in pier, correspondingly. Therefore, Rana cancrivora can be the indicator species for environment monitoring of mangrove.