CAJ | 학술논문

[目的]对泰山赤鳞鱼中酪氨酸酶的活性和表达差异进行研究。[方法]以泰山赤鳞鱼为材料,运用聚丙烯酰胺凝胶电泳与生化染色技术、分光光度技术和酶组织化学技术对赤鳞鱼雌、雄个体9种组织中的酪氨酸酶进行了分析研究。[结果]酪氨酸酶在雌、雄赤鳞鱼的肝胰脏、肠和脾组织中均有存在;同时在雄性赤鳞鱼的胆中也有分布。赤鳞鱼各组织中酪氨酸酶的活性大小存在较大差异。其中,雌雄性赤鳞鱼的脾脏中活性最强,但其活性低于雌性鱼脾中的酪氨酸酶活性。酶组织化学结果显示赤鳞鱼的脾脏、肠、肝胰脏和胆中都存在较强的酪氨酸酶阳性信号,而且脾中的阳性信号最强。[结论]该研究是关于泰山赤鳞鱼酪氨酸酶组织定位研究的首次报道,为进一步了解赤鳞鱼酪氨酸酶的功能提供了理论依据。
[목적]대태산적린어중락안산매적활성화표체차이진행연구。[방법]이태산적린어위재료,운용취병희선알응효전영여생화염색기술、분광광도기술화매조직화학기술대적린어자、웅개체9충조직중적락안산매진행료분석연구。[결과]락안산매재자、웅적린어적간이장、장화비조직중균유존재;동시재웅성적린어적담중야유분포。적린어각조직중락안산매적활성대소존재교대차이。기중,자웅성적린어적비장중활성최강,단기활성저우자성어비중적락안산매활성。매조직화학결과현시적린어적비장、장、간이장화담중도존재교강적락안산매양성신호,이차비중적양성신호최강。[결론]해연구시관우태산적린어락안산매조직정위연구적수차보도,위진일보료해적린어락안산매적공능제공료이론의거。
[Objective] This study aimed to analyze tyrosinase activity and its expression in Varicorhinus macrolepis. [Method] V. macrolepis was used as experimental material for the analysis and research of tyrosinase in nine kinds of organs and tissues of male and female V. macrolepis individuals by using polyacrylamide gel electrophoresis and biochemical staining method, spectrophotometry and enzyme histochemical technology. [Result] Tyrosinase exists in the liver and pancreas, intestine and spleen of female and male V. macrolepis and in the gallbladder of male V. macrolepis. Tyrosinase activities in various tissues of V. macrolepis varied largely. Specifically, tyrosinase activities in the spleen was the maximum, which was higher in female V. macrolepis than in males. According to the enzyme histochemistry results, strong positive signals of tyrosinase existed in the spleen, intestine, liver and pancreas and gallbladder of V. macrolepis, which was the strongest in the spleen. [Conclusion] In this paper, research on tissue localization of tyrosinase in V. macrolepis had been first reported, which provided theoretical basis for further exploring the functions of tyrosinase in V. macrolepis.