CAJ | 학술논문

[目的]探索活性污泥胞外聚合物（EPS）提取的最佳条件。[方法]比较研究了5种方法（H2SO4法、甲醛-NaOH法、搅拌法、热提法和NaOH法）对活性污泥EPS的提取效果,同时确定了最适提取方法的最优提取条件。[结果]5种提取方法中NaOH法因其提取的DNA含量较少,提取时间较短进而比较适宜。以NaOH法提取EPS,在pH为11,提取时间为10min,搅拌速度在80~120r/min下提取效率最高。[结论]确定的EPS最适提取条件可为其研究提供理论基础。
[목적]탐색활성오니포외취합물（EPS）제취적최가조건。[방법]비교연구료5충방법（H2SO4법、갑철-NaOH법、교반법、열제법화NaOH법）대활성오니EPS적제취효과,동시학정료최괄제취방법적최우제취조건。[결과]5충제취방법중NaOH법인기제취적DNA함량교소,제취시간교단진이비교괄의。이NaOH법제취EPS,재pH위11,제취시간위10min,교반속도재80~120r/min하제취효솔최고。[결론]학정적EPS최괄제취조건가위기연구제공이론기출。
[Objective] To explore the optimal extraction conditions of extracellular polymeric substances （EPS） from activated sludge. [Method] The efficiency of five methods （H2SO4, formaldehyde-NaOH, mixing, heating and NaOH） on the extraction of EPS was investigated comparatively. The optimal extraction conditions of the most suitable method were determined. [Result] NaOH method is most effective in extracting EPS with less DNA contamination and shortened extraction period. The optimal extraction condition was pH of 11, extraction time of 10 min and agitation speed of 80-120 r/min. [Conclusion] The determined optimal extraction condition provided theoretical basis for EPS study.