CAJ | 학술논문

建立了一种简单、实用的鸡蛋中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ残留的凝胶渗透色谱-超高效液相色谱检测方法。样品用乙腈∶丙酮=9∶1（V/V）溶液提取,经凝胶渗透色谱（GPC）净化后,反相液相色谱分离测定。本方法的检测限为20μg/kg,定量限为50μg/kg。四种苏丹红染料在20～2000 ng/mL范围内呈线性相关。在空白鸡蛋中添加50～1000μg/kg浓度下,四种苏丹红的平均回收率为65%～85%,批内变异系数在1.0%～4.8%之间（n=5）,批间变异系数在2.1%～5.9%之间（n=3）。结果表明,该法简单、灵敏、特异性强、重现性好,适用于鸡蛋中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ残留的测定。
건립료일충간단、실용적계단중소단홍Ⅰ、Ⅱ、Ⅲ、Ⅳ잔류적응효삼투색보-초고효액상색보검측방법。양품용을정∶병동=9∶1（V/V）용액제취,경응효삼투색보（GPC）정화후,반상액상색보분리측정。본방법적검측한위20μg/kg,정량한위50μg/kg。사충소단홍염료재20～2000 ng/mL범위내정선성상관。재공백계단중첨가50～1000μg/kg농도하,사충소단홍적평균회수솔위65%～85%,비내변이계수재1.0%～4.8%지간（n=5）,비간변이계수재2.1%～5.9%지간（n=3）。결과표명,해법간단、령민、특이성강、중현성호,괄용우계단중소단홍Ⅰ、Ⅱ、Ⅲ、Ⅳ잔류적측정。
Gel permeation chromatography-ultra-performance liquid chromatography method for simultaneous determination of sudanⅠ、Ⅱ、Ⅲ and Ⅳ was developed.The samples were extracted by acetonitrile： aceton（9∶ 1）,cleaned up by gel permeation chromatography,and determined by ultra-performance liquid chromatography.The limit of detection was 20 μg/kg.The limit of quantification was 50 μg/kg.The calibration curves of the four sudan dyes were linear over the concentration ranges of 20~2000 ng/mL.The average recoveries ranged from 65% to 85%.The inter-run relative standard deviation（RSD） was in the range of 1.0%~4.8%（n=5）,and the between-run relative standard deviation（RSD） was in the range of 2.1%~5.9%（n=3）.Because of its high sensitive,good precision and specificity,the method was found to be suitable for trace analysis of sudans in eggs.