CAJ | 학술논문

采用HPLC-DAD-MSn法对兔肌注恩诺沙星给药后血浆中恩诺沙星和代谢产物的紫外光谱和质谱裂解行为进行分析,获得各化合物的光谱和多级质谱信息。比较给药前后兔血浆的DAD检测色谱图,发现6个可能的代谢产物M1……M6。根据化合物结构和质谱裂解途径及标准品的色谱保留时间,确定M3为环丙沙星,并推测M5可能为加氧恩诺沙星;M4获得一级质谱数据和紫外光谱数据;M1、M2和M6仅得到紫外光谱数据。推测恩诺沙星在兔体内的主要代谢反应是脱乙基反应和氧化反应。
채용HPLC-DAD-MSn법대토기주은낙사성급약후혈장중은낙사성화대사산물적자외광보화질보렬해행위진행분석,획득각화합물적광보화다급질보신식。비교급약전후토혈장적DAD검측색보도,발현6개가능적대사산물M1……M6。근거화합물결구화질보렬해도경급표준품적색보보류시간,학정M3위배병사성,병추측M5가능위가양은낙사성;M4획득일급질보수거화자외광보수거;M1、M2화M6부득도자외광보수거。추측은낙사성재토체내적주요대사반응시탈을기반응화양화반응。
The enrofloxacin and its metabolites in the plasma of rabbit following intramuscular injection, were analyzed using HPLC integrated diode array detector（DAD） and electrospray ion （ESI） trap mass spectrometry in a multistage MS full scan mode. Six potential metabolites, M1 to M6 were found by comparing the chromatogram of DAD of the plasma in rabbits before and post injecting enrofloxacin, which have similar ultraviolet spectrum to enrofloxacin. The multistage mass spectral information was obtained following the mass spectrometric analysis of enrofloxacin and its metabolites （M3 and M5 ） by collision induced dissociation. The result showed that enrofloxacin was biotransformed to six metabolites in the plasma. In which, the structure of two metabolites, ciprofloxacin was identified and oxyenrofloxacin was speculated. The main mechanism of biotransformed for enrofloxacin may be dethylation and oxidation effects.