CAJ | 학술논문

为筛选出川贝母组织培养物中最适于用玻璃化法进行超低温保存的材料及其最适的玻璃化溶液脱水处理时间，将继代15d的优质川贝母芽、愈伤组织和鳞茎接入Ms＋30g／L蔗糖＋5％二甲基亚砜的固体培养基中预培养6d后，用60％玻璃化溶液Ⅱ常温装载25min，再于2℃下用100％玻璃化溶液11分别进行不同时间的脱水处理后投入液氮中保存．结果显示，在玻璃化超低温保存过程中，川贝母芽、愈伤组织和鳞茎的最佳脱水处理时间均为60win，其中愈伤组织细胞保存后的相对存活率最高为80．08％．
위사선출천패모조직배양물중최괄우용파리화법진행초저온보존적재료급기최괄적파리화용액탈수처리시간，장계대15d적우질천패모아、유상조직화린경접입Ms＋30g／L자당＋5％이갑기아풍적고체배양기중예배양6d후，용60％파리화용액Ⅱ상온장재25min，재우2℃하용100％파리화용액11분별진행불동시간적탈수처리후투입액담중보존．결과현시，재파리화초저온보존과정중，천패모아、유상조직화린경적최가탈수처리시간균위60win，기중유상조직세포보존후적상대존활솔최고위80．08％．
To filter the most appropriate cultured Fritillaria cirrhosa D. Don tissue for cryopreservation by vitrification and its best dehydration treatment time bud, bulbs and callus of cultured Fritillaria cirrhosa D. Don which have been subcultured for 15 days and precultured in solid medium MS ＋ 30 g/L sucrose ＋ 5 % DMSO for 6 days were loaded with 60% PVS2 for 25 min under room temperature, and dehydrated with 100% PVS2 for different time at 2 ℃ respectively, then put into the liquid nitrogen for preservation. The results show that the best dehydration treatment time of bud, bulbs and callus of cultured Fritillaria cirrhosa D. Don all are 60 min, and the highest survival rate of callus reaches 80.08 %.