CAJ | 학술논문

登革病毒依抗原性不同,可分为1、2、3、4四个血清型。它除了能导致一定范围内发热的典型登革热外,还能引致更严重的登革出血热和登革休克综合症。由于没有具体有效的治疗药物,目前尚无好的治疗登革热的方法,治疗方案多是支持性的。本实验评估了在2型登革病毒感染的人血源性巨噬细胞中沙棘叶提取物的抗登革病毒活性,选择巨噬细胞的原因是因为巨噬细胞是登革病毒感染的首要目标。受感染的细胞用沙棘叶提取物治疗并与市售的抗病毒药物利巴韦林作比较,研究发现沙棘叶提取物维持登革病毒感染细胞活力的能力几乎等同于利巴韦林。传统的空斑试验进一步确定了沙棘叶提取物的抗登革病毒活性。这些试验结果表明,沙棘叶提取物具有显著的抗登革病毒活性及治疗登革热的潜力。
등혁병독의항원성불동,가분위1、2、3、4사개혈청형。타제료능도치일정범위내발열적전형등혁열외,환능인치경엄중적등혁출혈열화등혁휴극종합증。유우몰유구체유효적치료약물,목전상무호적치료등혁열적방법,치료방안다시지지성적。본실험평고료재2형등혁병독감염적인혈원성거서세포중사극협제취물적항등혁병독활성,선택거서세포적원인시인위거서세포시등혁병독감염적수요목표。수감염적세포용사극협제취물치료병여시수적항병독약물리파위림작비교,연구발현사극협제취물유지등혁병독감염세포활력적능력궤호등동우리파위림。전통적공반시험진일보학정료사극협제취물적항등혁병독활성。저사시험결과표명,사극협제취물구유현저적항등혁병독활성급치료등혁열적잠력。
Dengue virus occurs as four distinct serotypes,called Dengue 1,2,3,and 4.Symptomaticdengue virus infection ranges from a self limited febrile illness,dengue fever（DF）,to a more severedisease,dengue hemorrhagic fever/dengue shock syndrome（DHF/DSS）.The anti-Dengue treatmentis severely hampered as no specific therapeutic agents are available.Even present treatmentstrategies for Dengue are more supportive than curative.In the present study anti-dengue activity of Hippoplzae rhamnoides{Seabuckthorn,SBT）leaf extract 4vas evaluated in Dengue virus type-2 infected blood-derived human macrophages as macrophages are the primary target of Denguevirus infection.Infected cells were treated with SBT leaf extract and compared with commerciallyavailable anti-viral drug,Ribavirin.The extract was able to maintain the cell viability of Dengue-infected cells at par with Ribavirin along with the decrease and increase in TNF-[alpha]and IFN-[gamma]respectively.Anti-dengue activity of SBT extract was further determined by the traditionalplaque assay.These observations suggest that the SBT leaf extract has a significant anti-dengueactivity and has the potential for the treatment of Dengue.