现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2012年
4期
151-152
,共2页
汪云%罗富英%李典%李路金%蓝素华
汪雲%囉富英%李典%李路金%藍素華
왕운%라부영%리전%리로금%람소화
紫色马铃薯%幼芽%快速繁殖%外源激素
紫色馬鈴藷%幼芽%快速繁殖%外源激素
자색마령서%유아%쾌속번식%외원격소
purple potato(Solanum tuberosum L.)%plumule%micropropagation%exohormone
以幼嫩紫色马铃薯芽为试验材料,探讨不同激素组合对紫色马铃薯芽快速繁殖生长情况的影响,并成功获得一批无菌苗。结果表明：以75%酒精（CH3CH2OH）、0.1%升汞分别灭菌10 s、8 min,外植体诱导出芽数最高可达23个,长势良好。紫色马铃薯芽诱导培养基为MS＋0.5 mg/L 6-BA＋0.3 mg/L NAA＋30 g/L蔗糖＋7 g/L琼脂,芽分化成苗率为57.50%,茎粗壮,长势整齐,苗高5.12 cm,生根率达60.00%。
以幼嫩紫色馬鈴藷芽為試驗材料,探討不同激素組閤對紫色馬鈴藷芽快速繁殖生長情況的影響,併成功穫得一批無菌苗。結果錶明：以75%酒精（CH3CH2OH）、0.1%升汞分彆滅菌10 s、8 min,外植體誘導齣芽數最高可達23箇,長勢良好。紫色馬鈴藷芽誘導培養基為MS＋0.5 mg/L 6-BA＋0.3 mg/L NAA＋30 g/L蔗糖＋7 g/L瓊脂,芽分化成苗率為57.50%,莖粗壯,長勢整齊,苗高5.12 cm,生根率達60.00%。
이유눈자색마령서아위시험재료,탐토불동격소조합대자색마령서아쾌속번식생장정황적영향,병성공획득일비무균묘。결과표명：이75%주정（CH3CH2OH）、0.1%승홍분별멸균10 s、8 min,외식체유도출아수최고가체23개,장세량호。자색마령서아유도배양기위MS＋0.5 mg/L 6-BA＋0.3 mg/L NAA＋30 g/L자당＋7 g/L경지,아분화성묘솔위57.50%,경조장,장세정제,묘고5.12 cm,생근솔체60.00%。
In this experiment,purple potato（Solanum tuberosum L.）was used as the experimental material.Studing on the concept of micropropagation of purple potato bud expant,and some cultural plantlets were obtained successfully.The experimental results indicated that separately using 75% CH3CH2OH（8 min） and 0.1% HgCI2（10 s） to sterilize the loseeonds,the average number of buds up to 23,and the buds grow well.The buds induction medium of purple potato was MS＋0.5mg/L 6-BA ＋0.3 mg/L NAA ＋30 g/L sucros ＋7 g/L agar.The rate of buds differentiation was 57.50%,the plantlets growed thick and strong,and the sprout growed evenly.The height of plant was 5.12 cm,and the rate of root growing reached to 60.00%.
