安徽农学通报
安徽農學通報
안휘농학통보
AUHUI AGRICULTURAL SCIENCE BULLETIN
2012年
13期
47-49
,共3页
解磷菌筛选%钼锑抗比色法%培养特性
解燐菌篩選%鉬銻抗比色法%培養特性
해린균사선%목제항비색법%배양특성
Isolation of phosphate solubilizing bacteria%Solybdenum stibium anti - color method%Culture characteristic
采用限制性培养的方法从大庆多年耕作的农田土壤中分离筛选出的无机磷降解菌1株。在以Ca3（PO4）2为唯一磷源的平板培养中对其解磷能力进行测定,结果能观察到溶磷圈,但溶磷圈直径较小;在液体培养法中利用钼锑抗比色法对其解磷能力进行测定,在以Ca3（PO4）2为唯一磷源的培养液中的可溶性磷得率为10.01%,解磷能力较强。通过研究不同初始pH、温度、气液比对解磷能力的影响,确定分解Ca3（PO4）2的最佳条件为30℃,180r/min,pH7～8。气液比越小越有利于溶磷。
採用限製性培養的方法從大慶多年耕作的農田土壤中分離篩選齣的無機燐降解菌1株。在以Ca3（PO4）2為唯一燐源的平闆培養中對其解燐能力進行測定,結果能觀察到溶燐圈,但溶燐圈直徑較小;在液體培養法中利用鉬銻抗比色法對其解燐能力進行測定,在以Ca3（PO4）2為唯一燐源的培養液中的可溶性燐得率為10.01%,解燐能力較彊。通過研究不同初始pH、溫度、氣液比對解燐能力的影響,確定分解Ca3（PO4）2的最佳條件為30℃,180r/min,pH7～8。氣液比越小越有利于溶燐。
채용한제성배양적방법종대경다년경작적농전토양중분리사선출적무궤린강해균1주。재이Ca3（PO4）2위유일린원적평판배양중대기해린능력진행측정,결과능관찰도용린권,단용린권직경교소;재액체배양법중이용목제항비색법대기해린능력진행측정,재이Ca3（PO4）2위유일린원적배양액중적가용성린득솔위10.01%,해린능력교강。통과연구불동초시pH、온도、기액비대해린능력적영향,학정분해Ca3（PO4）2적최가조건위30℃,180r/min,pH7～8。기액비월소월유리우용린。
A strain of phosphate solubilizing bacteria was chosen by the method of the restrictive culture from farmland soil being cultivated many years in Daqing. The degradation ability of the bacterium in medium taking Ca3 （ PO4 ） 2 as the only phosphate source showed the phosphate circle could be observed and the phosphate circle diameter was too small. Results showed that the phosphate solubilizing efficiency of the strain was up to 10.01% when with Ca3 （PO4）2 as the sole phosphor- us source in the medium. By studying the effect of degeneration ability on different initial pH, temperature, gas fluid ratio, the result showed that optimum conditions were 30℃, 180r/min, pH7 -8, and the low fluid ratio was benefit to phosphate solubi- lizing.
