CAJ | 학술논문

试验选用新疆野核桃叶为研究对象,以金黄色葡萄球菌为指示菌,用抑菌圈直径为指标,在单因素试验的基础上通过正交实验对核桃叶的提取条件进行优化。结果表明：对核桃叶抑菌活性物质提取效果的影响为乙醇浓度〉液固比〉提取温度〉提取时间;最佳提取条件：乙醇浓度70%,液固比15∶1,提取温度60℃,提取时间4h。在此优化条件下,核桃叶提取物抑菌圈直径为1.932cm。
시험선용신강야핵도협위연구대상,이금황색포도구균위지시균,용억균권직경위지표,재단인소시험적기출상통과정교실험대핵도협적제취조건진행우화。결과표명：대핵도협억균활성물질제취효과적영향위을순농도〉액고비〉제취온도〉제취시간;최가제취조건：을순농도70%,액고비15∶1,제취온도60℃,제취시간4h。재차우화조건하,핵도협제취물억균권직경위1.932cm。
This experiment through wild walnut leaf extract, confirmed the vitro antimicrobial test of wild walnut leaf extract gram- positive bacteria have obvious antibacterial function. Xinjiang wild walnut leaf test for researching object,by staphy- lococcus aureus, with instructions for the bacteriostatic circle diameter of activity tracking, on the basis of single factor experi- ment through orthogonal experiment of walnut leaf extract optimized conditions. As Results of walnut leaves the antibacterial activity material extraction effect size order： Ethanol concentration 〉 The liquid - solid ratio 〉 Extracting temperature 〉 Ex- tracting time;The best condition： ethanol concentration70%, The liquid- solid ratio15： 1, Extracting temperature60℃, Ex- tracting time 4 hours o In the optimized conditions, walnut leaf extract bacteriostatic circle for 1. 932 cm in diameter.